تأثیر پیش‌تیمار بذر بر خصوصیات رشدی، عملکرد و فعالیت آنزیمی گوجه‌فرنگی در شرایط تنش سرما

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد باغبانی، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران

2 دانشیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران

3 استادیار گروه علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران

4 استادیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، د انشگاه فردوسی مشهد، مشهد، ایران

5 استادیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران

چکیده

به‌منظور بررسی تأثیر پیش‌تیمار بذر بر شاخص‌های رشدی و عملکرد میوه گوجه‌فرنگی در شرایط دمای پایین، آزمایشی به‎صورت آزمایشگاهی و گلخانه‌ای در گروه علوم باغبانی دانشکده علوم کشاورزی دانشگاه محقق اردبیلی در سال 1391 اجرا شد. برای این منظور گیاهچه‌های گوجه‌فرنگی حاصل از بذرهای پیش تیمار شده به ترتیب با غلظت‌های 5/1، 5/0 و 5/0 میلی‌مولار از پلی‌آمین‌های اسپرمین، اسپرمیدین و پوترسین و 50 میلی‌گرم در لیتر متیل‌جاسمونات در سه روز متوالی و هر روز به مدت 4 ساعت در معرض دمای 4 درجه‌سانتی‌گراد قرار گرفتند. صفات مورد مطالعه شامل: قطر ساقه، ارتفاع بوته، تعداد روز تا گلدهی، تعداد گره گل‌دهنده، زمان گلدهی و میوه‌دهی، هدایت روزنه‌ای و محتوای کلروفیل برگ، تعداد میوه، میانگین قطر میوه، وزن کل میوه، تعداد برگ، تعداد گره، طول ساقه، قطر ساقه، سطح برگ، وزن خشک ریشه و وزن خشک میوه گوجه‌فرنگی، محتوای پروتئین کل و فعالیت آنزیم‌های پراکسیداز و کاتالاز بودند. نتایج نشان داد تأثیر پیش‌تیمار بذر بر محتوای کلروفیل برگ، زمان میوه‌دهی، روز تا گلدهی، گره گلدهنده، تعداد خوشه، تعداد میوه، قطر میوه، وزن تر و خشک میوه و میزان فعالیت آنزیم پراکسیداز گوجه‌فرنگی معنا‌دار بود. تنش سرما فلورسانس حداقل و حداکثر و قطر ساقه را به طور معناداری تحت تأثیر قرار داد. اثر متقابل پیش‌تیمار بذر و تنش سرما بر قطر ساقه و درصد ماده خشک اندام هوایی معنا‌دار شد. پیش‌تیمار بذر باعث کاهش معنا‌دار زمان گلدهی گوجه‌فرنگی شد، به طوری‌که بیشترین زمان گلدهی در تیمار شاهد 5/89 روز و کمترین زمان 81 روز برای پیش‌تیمار با اسپرمیدین حاصل شد.    

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Seed Pre-treatment on Growth Characteristics, Yield and Enzymatic Activity of Tomato under Cold Stress Conditions

نویسندگان [English]

  • Mohammad Norouzi Givi 1
  • Behrooz Esmaielpour 2
  • Mehdi Moheb Al-Dini 3
  • Seror Khoramdel 4
  • Saeed Khomari 5
1 MS.c. Student, Department of Horticulture Sciences, Faculty of Agriculture, University of Mohaghegh Ardebil, Ardebil, Iran
2 Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardebil, Iran
3 Assisant Professor, Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
4 Assistant Professor, Department of Agriculture and Plant breeding , Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
5 Assisant Professor, Department of Agriculture and plant breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده [English]

In order to investigate the effect of seed pretreatment on growth indices and tomato fruit yield in low temperature conditions, an experimental laboratory and greenhouse experiment was conducted in the Department of Horticulture, Faculty of Agricultural Sciences, Mohaghegh Ardabili University in 2012. For this purpose, tomato seedlings obtained from pretreated seeds with concentrations of 1.5, 0.5 and 0.5 mmol of polyamines spermine, spermidine and putrescine and 50 mg / l methyl jasmonate for three consecutive days and daily for 4 days, respectively. The clocks were exposed to 4 ° C. Study traits include: stem diameter, plant height, number of days to flowering, number of flowering nodes, flowering and fruiting time, stomatal conductance and leaf chlorophyll content, number of fruits, average fruit diameter, total fruit weight, number of leaves, number of nodes, length Stem, stem diameter, leaf area, root dry weight and tomato fruit dry weight, total protein content and activity of peroxidase and catalase enzymes. The results showed that the effect of seed pretreatment on leaf chlorophyll content, fruiting time, day to flowering, flowering node, number of spikes, number of fruits, fruit diameter, fresh and dry weight of fruit and tomato peroxidase activity was significant. Cold stress significantly affected the minimum and maximum fluorescence and stem diameter. The interaction effect of seed pretreatment and cold stress on stem diameter and shoot dry matter percentage was significant. Seed pretreatment significantly reduced the flowering time of tomatoes, so that the maximum flowering time in the control treatment was 89.5 days and the minimum time was 81 days for pretreatment with spermidine.

کلیدواژه‌ها [English]

  • Spermidine
  • Polyamines
  • Number of fruits
  • Methyl jasmonate
  • Stomatal conductance
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