تأثیر مدت زمان تابش نور و کلسیم بر رشد، عملکرد و کیفیت کاهو رقم New-Red Fire

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

2 دانشیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

3 استادیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

10.22034/iuvs.2021.521006.1135

چکیده

به‌منظور بررسی تأثیر کلسیم و طول مدت تابش نور بر رشد و کیفیت کاهو رقم New-Red Fire، آزمایشی به‌صورت کرت‌های خرد شده در قالب طرح پایه کاملاً تصادفی با سه تکرار در گلخانه تحقیقاتی دانشگاه زنجان در سال 1398 انجام شد. فاکتور ‌اصلی شامل طول مدت تابش نور در دو سطح طول‌ مدت تابش ‌طبیعی (به‌طور میانگین 7000 تا 8000 لوکس) و طول ‌مدت تابش‌ طبیعی به‌علاوه چهار ساعت نور مکمل (600 لوکس) و فاکتور فرعی محلول‌پاشی برگی کلسیم شامل سه سطح لاکتات‌ کلسیم (5/0، یک و 5/1 گرم در لیتر) و سه سطح کلرید کلسیم (سه، شش و نُه گرم در لیتر) به همراه شاهد (آب مقطر) بودند. نتایج نشان ‌داد که افزایش مدت تابش نور تعداد برگ، وزن تر بوته و محتوای کلروفیل، فنول و فلاونوئید، ویتامین ث و آنتوسیانین را به‌طور معنی‌داری افزایش داد. کاربرد شش و نُه گرم در لیتر کلرید کلسیم و 5/1 گرم در لیتر لاکتات کلسیم، وزن تر بوته (3/10، 5/10 و 6/11 گرم) و غلظت کلسیم برگ‌های داخلی (8/12، 9/12 و 2/12 درصد) کاهو را افزایش داد و از ظهور عارضه نوک سوختگی برگ جلوگیری کرد. بیشترین تعداد برگ‌های سوخته (3 برگ) در گیاهان شاهد تحت تیمار نور مکمل مشاهده شد. همچنین حداکثر مقدار وزن تر بوته، محتوای آنتوسیانین، فنول، فلاونوئید کل و فعالیت آنتی‌اکسیدانی با کاربرد 5/1 گرم در لیتر لاکتات کلسیم، شش و نُه گرم در لیتر کلرید کلسیم به همراه افزایش نور به‌دست ‌آمد. با توجه به نتایج محلول‌پاشی برگی 5/1 گرم در لیتر لاکتات ‌کلسیم، شش و نُه گرم در لیتر کلرید کلسیم به همراه افزایش مدت تابش نور جهت بهبود رشد و کیفیت کاهو پیشنهاد می‌گردد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Light Duration and Calcium on Growth, Yield and Quality of Lettuce (Lactuca sativa cv. New Red Fire)

نویسندگان [English]

  • Hafizullah Niazi 1
  • Taher Barzegar 2
  • Zahra Ghahremani 3
  • Leila Nadirkhanlou 1
1 M.Sc. Graduate, Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2 Associate Professor, Department of Horticulture Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
3 Assistant Professor, Department of Horticulture Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
چکیده [English]

In order to study the effects of calcium and light duration on growth and quality of lettuce, this experiment was conducted as split plot based on the randomized complete design with three replications, in research greenhouse of University of Zanjan during 2020. The main plot consisted of light treatments at two levels included natural light duration (an average 7000-8000 lux) and natural light in addition four hourse complementary light (600 lux) and the subplot was consisted of three levels of calcium lactate (0.5, 1 and 1.5 g L-1(, calcium chlorid (3, 6 and 9 g.L-1) and distilled water as a control. The results showed that increasing light duration significantly increased leaves number, plant fresh weight, total chlorophyll, phenols and flavonoids, vitamin C and anthocyanin contents. Application of calcium chlorid (6 and 9 g.L-1) and calcium lactate (1.5 g.L-1( increased plant fresh weight (10.3, 10.5 and 11.6 g) and Ca concentration of internal leaves (12.8, 12.9 and 12.2%) lettuce, and inhibiet tip burn disorder in lettuce lives. The highest tip burn leaf number (3.5) was observed in plants whitout Ca teated under complementary ligh treatment. Also the highest amount of plant fresh weight, anthocyanin, total phenol and flavonoids and antioxidant capacity was obtained by using calcium lactate 1.5 and calcium chloride 3 and 6 g.L-1 with increasing light duration. According to the results, application of calcium lactate 1.5 g.L-1 and calcium chlorid 6 and 9 g.L-1 with increasing light duration can be preposed to improve growth and quality of lettuce.

کلیدواژه‌ها [English]

  • anthocyanin
  • Chlorophyll
  • fresh weight
  • Tipburn
  • Vitamin C
-          Aguayo, E., Requejo-Jackman, C., Stanley, R. & Woolf, A. (2010). Effects of calcium ascorbate treatments and storage atmosphere on antioxidant activity and quality of fresh-cut apple slices. Postharvest Biology and Technology, 57(1), 52-60.‏
-          Ahmed, M. J., Singh, Z. & Khan, A. S. (2009). Postharvest Aloe vera gel‐coating modulates fruit ripening and quality of ‘Arctic Snow’nectarine kept in ambient and cold storage. International Journal of Food Science & Technology, 44(5), 1024-1033.‏
-          Akhtar, A., Abbasi, N. A. & Hussain, A. Z. H. A. R. (2010). Effect of calcium chloride treatments on quality characteristics of loquat fruit during storage. Pakistan Journal of Botany, 42(1), 181-188.‏
-          Ali, M. B., Khandaker, L. & Oba, S. (2009). Comparative study on functional components, antioxidant activity and color parameters of selected colored leafy vegetables as affected by photoperiods. Journal of Food and Agriculture and Environment, 7(3), 392-398.‏
-          AOAC. (2000). Official methods of analysis. 17th Edition, the Association of Official Analytical Chemists, Gaithersburg, MD, USA.
-          Baroli, I., Price, G. D., Badger, M. R. & von Caemmerer, S. (2008). The contribution of photosynthesis to the red light response of stomatal conductance. Plant Physiology, 146(2), 737-747.‏
-          Barzegar, T., Fateh, M. & Razavi, F. (2018). Enhancement of postharvest sensory quality and antioxidant capacity of sweet pepper fruits by foliar applying calcium lactate and ascorbic acid. Scientia Horticulturae, 241, 293-303.‏
-          Bian, Z., Yang, Q. C. & Liua, W. K. (2015). Effects of light quality on the accumulation of phytochemicals in vegetables produce in controlled environments: a review. Food Science & Technology, 95(5), 869-877.
-          Borghesi, E., Carmassi, G., Uguccioni, M. C., Vernieri, P. & Malorgio, F. (2013). Effects of calcium and salinity stress on quality of lettuce in soilless culture. Journal of Plant Nutrition, 36(5), 677-690.‏
-          Chang, A. C., Yang, T. Y. & Riskowski, G. L. (2013). Ascorbic acid, nitrate, and nitrite concentration relationship to the 24 hour light/dark cycle for spinach grown in different conditions. Food Chemistry, 138(1), 382-388.‏
-          Chang, C. C., Yang, M. H., Wen, H. M. & Chern, J. C. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3), 1-7.‏
-          Corriveau, J., Gaudreau, L., Caron, J., Gosselin, A., & Jenni, S. (2010). Effect of water management, fogging and Ca foliar application on tipburn of Romaine lettuce (Lactuca sativa L.) cultivated in greenhouse. In XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on 927. 475-480.‏
-          Fan, X. X., Xu, G. Z., Liu, X. Y., Tang, C. M., Wang, L. W. & Han, X. L. (2013). Effects of light intensity on the growth and leaf development of young tomato plants grown under a combination of red and blue light. Scientia Horticulturae, 153, 50-55.
-          FAOSATE. (2018). Food and Agriculture Organization of the United Nations, FAO data base.
-          Frezza, D., Leon, A., Logegaray, V., Chiesa, A., Desimone, M. & Diaz, L. (2005). Soilless culture technology for high quality lettuce. Acta Horticulturae, 697, 43-48.‏
-          Gorgini Shabankareh, H. & Fakheri, B. (2015). The effect of different levels of salinity and drought stresses on growth indices and the essential oil of lemon balm (Melissa officinalis L.). Iranian Jurnal of Field Crop Science, 46(4), 686-673.‏
-          He, L., Yu, L., Li, B., Du, N. & Guo, S. (2018). The effect of exogenous calcium on cucumber fruit quality, photosynthesis, chlorophyll fluorescence, and fast chlorophyll fluorescence during the fruiting period under hypoxic stress. BMC Plant Biology, 18(1), 1-10.‏
-          Heo, J. W., Kang, D. H., Bang, H. S., Hong, S. G., Chun, C. & Kang, K. K. (2012). Early growth, pigmentation, protein content, and phenylalanine ammonia-lyase activity of red curled lettuces grown under different lighting conditions. Korean Journal of Horticultural Science & Technology, 30, 6-12.
-          Hepler, P. K. (2005). Calcium: a central regulator of plant growth and development. The Plant Cell, 17(8), 2142-2155.‏
-          Holb, I. J., Balla, B., Vamos, A. & Gall, J. M. (2012). Influence of preharvest calcium applications, fruit injury, and storage atmospheres on postharvest brown rot of apple. Postharvest Biology and Technology, 67, 29-36.‏
-          Johkan, M., Shoji, K., Goto, F., Hashida, S. N. & Yoshihara, T. (2010). Blue light-emitting diode light irradiation of seedlings improves seedling quality and growth after transplanting in red leaf lettuce. Horticulturea Science, 45(12), 1809-1814.‏
-          Kalra, Y. (1997). Handbook of reference methods for plant analysis. London. CRC press.
-          Kang, J. H., KrishnaKumar, S., Atulba, S. L. S., Jeong, B. R. & Hwang, S. J. (2013). Light intensity and photoperiod influence the growth and development of hydroponically grown leaf lettuce in a closed-type plant factory system. Horticulture, Environment, and Biotechnology, 54(6), 501-509.‏
-          Karimi, E., Jaafar, H. Z., Ghasemzadeh, A. & Ibrahim, M. H. (2013). Light intensity effects on production and antioxidant activity of flavonoids and phenolic compounds in leaves, stems and roots of three varieties of Labisia pumila Benth. Australian Journal of Crop Science, 7(7), 1016-1023.
-          Kaya, C., Kirnak, H., Higgs, D. & Saltali, K. (2002). Supplementary calcium enhances plant growth and fruit yield in strawberry cultivars grown at high (NaCl) salinity. Scientia Horticulturae, 93(1), 65-74.‏
-          Kazemi, M., Aran, M. & Zamani, S. (2011). Effect of calcium chloride and salicylic acid treatments on quality characteristics of kiwifruit (Actinidia deliciosa cv. Hayward) during storage. American Journal of Plant Physiology, 6(3), 183-189.‏
-          Khani, A., Barzegar, T., Nikbakht, J. & Ghahremani, Z. (2020). Effect of foliar spray of calcium lactate on the growth, yield and biochemical attribute of lettuce (Lactuca sativa L.) under water deficit stress. Advances in Horticultural Science, 34(1), 11-24.‏
-          Khani, A., Barzegar. T., Ghahremani, Z. & Nikbakht. J. (2019). The effect of calcium lactate foliar spray on growth, yield and quality of Lettuce cv. New Red Fire under water deficit stress. Journal of Plant Process and Function, 8(33), 187-202. (In Farsi)
-          Kou, L., Yang, T., Liu, X. & Luo, Y. (2015). Effects of pre-and postharvest calcium treatments on shelf life and postharvest quality of broccoli microgreens. Horticultural Science, 50(12), 1801-1808.‏
-          Kozai, T., Niu, G. & Takagaki, M. (2016). Plant factory. Academic press, London, UK.
-          Kwaw, E., Ma, Y., Tchabo, W., Apaliya, M. T., Sackey, A. S., Wu, M. & Xiao, L. (2018). Impact of ultrasonication and pulsed light treatments on phenolics concentration and antioxidant activities of lactic-acid-fermented mulberry juice. LWT -Food Science and Technology, 92, 61-66.‏
-          Li, H., Tang, C., Xu, Z., Liu, X. & Han, X. (2012). Effects of different light sources on the growth of non-heading Chinese cabbage (Brassica campestris L.). Journal of Agricultural Science, 4(4), 262.‏
-          Li, Q. & Kubota, C. (2009). Effects of supplemental light quality on growth and phytochemicals of baby leaf lettuce. Environmental and Experimental Botany, 67(1), 59-64.‏
-          Llorach, R., Martinez-Sanchez, A., Tomas-Barberan, F. A., Gil, M. I. & Ferreres, F. (2008). Characterisation of polyphenols and antioxidant properties of five lettuce varieties and escarole. Food Chemistry, 108(3), 1028-1038.‏
-          Madani, B., Mirshekari, A. & Yahia, E. (2015). Effect of calcium chloride treatments on calcium content, anthracnose severity and antioxidant activity in papaya fruit during ambient storage. Journal of the Science of Food and Agriculcure,96, 2963-2968.
-          Mahajan, B. V. C. & Sharma, R. C. (2000). Effect of pre-harvest applications of growth regulators and calcium chloride on physico-chemical characteristics and storage life of peach (Prunus persica Batsch) cv. Shane-e-Punjab. Haryana Journal of Horticultural Sciences, 29(1), 41-43.‏
-          Merzlyak, M. N. & Chivkunova, O. B. (2000). Light-stress-induced pigment changes and evidence for anthocyanin photoprotection in apples. Journal of Photochemistry and Photobiology B: Biology, 55(2), 155-163.‏
-          Mohd, F. J., Shampazuraini, S., Mohammad, A. A. & Mohamad, N. J. (2015). Effects of different types of foliar fertilizer on growth performance of spider plant (Cleome gynandra). Australian Journal of Basic and Applied Sciences, 9(24), 44-48.‏
-          Moreira-Rodriguez, M., Nair, V., Benavides, J., Cisneros-Zevallos, L. & Jacobo-Velazquez, D. A. (2017). UVA, UVB light, and methyl jasmonate, alone or combined, redirect the biosynthesis of glucosinolates, phenolics, carotenoids, and chlorophylls in broccoli sprouts. International Journal of Molecular Sciences, 18(11), 2330.‏
-          Nishiyama, M. & Kanahama, K. (2008, March). Effect of light quality on growth of everbearing strawberry plants. In VI International Strawberry Symposium 842 (pp. 151-154).‏
-          Noctor, G. (2006). Metabolic signalling in defence and stress: the central roles of soluble redox couples. Plant, Cell & Environment, 29(3), 409-425.‏
-          Ohashi-Kaneko, K., Takase, M., Kon, N., Fujiwara, K. & Kurata, K. (2007). Effect of light quality on growth and vegetable quality in leaf lettuce, spinach and komatsuna. Environmental Control in Biology, 45(3), 189-198.‏
-          Rab, A. & Haq, I. U. (2012). Foliar application of calcium chloride and borax influences plant growth, yield, and quality of tomato (Lycopersicon esculentum Mill.) fruit. Turkish Journal of Agriculture and Forestry, 36(6), 695-701.‏
-          Rogers, G. S. (2007). Development of a crop management program to improve the sugar-content and quality of rockmelons. Horticultural Australia.‏
-          Roussos, P. A., Denaxa, N. & Damvakaris, T. (2009). Strawberry fruit quality attributes after application of plant growth stimulating compounds. Scientia Horticulturae, 119(2), 138-146.‏
-          Sago, Y. (2016). Effects of light intensity and growth rate on tipburn development and leaf calcium concentration in butterhead lettuce. Horticultural Science, 51(9), 1087-1091.‏
-          Samuoliene, G., Brazaityte, A., Urbonavicute, A., Sabajeviene, G. & Duchovskis, P. (2010). The effect of red and blue light component on the growth and development of frigo strawberries. Zemdirbyste-Agriculture, 97(2), 99-104.‏
-          Saure, M. C. (2005). Calcium translocation to fleshy fruit: its mechanism and endogenous control. Scientia Horticulturae, 105(1), 65-89.‏
-          Shiri, M. A., Ghasemnezhad, M., Fatahi Moghadam, J. & Ebrahimi, R. (2016). Effect of CaC l2 sprays at different fruit development stages on postharvest keeping quality of “H ayward” kiwifruit. Journal of Food Processing and Preservation, 40(4), 624-635.‏
-          Singleton, V. L. & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, 16(3), 144-158.‏
-          Sun, T., .Powers, J. & Tang, J. (2007). Evaluation of the antioxidant activity of asparagus, broccoli and their juices. Food Chemistry, 105, 101-106.
-          Taiz, L. & Ezeiger, H. (2008). Plant physiology. (2nd Ed.). Sinaye Associates Inc. Publisher. Sonderland Massachusetts.
-          Takahashi, S. & Badger, M. R. (2011). Photoprotection in plants: a new light on photosystem II damage. Trends in Plant Science, 16(1), 53-60.‏
-          Tan, B. L., Norhaizan, M. E. & Liew, W. P. P. (2018). Nutrients and oxidative stress: friend or foe?. Oxidative Medicine and Cellular Longevity, 1-24.
-          Todd, A., Peterson, T. M., Blackmer, D. D., Francis, J. & Schepers, S. (2005). Using a chlorophyll meter to improve N management. Soil Science, 93, 1173-1177.
-          Toor, R. K., Savage, G. P. & Lister, C. E. (2006). Seasonal variations in the antioxidant composition of greenhouse grown tomatoes. Journal of Food Composition and Analysis, 19(1), 1-10.‏
-           Vrana, L. M. & Staff, U. (2014) Calcium Chloride. In Kirk-Othmer Encyclopedia of Chemical Technology, 1-13.
-          Wagner, G. J. (1979). Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts. Plant Physiology, 64(1), 88-93.‏
-          Wanlai, Z., Wenke, L. & Qichang, Y. (2013). Reducing nitrate content in lettuce by pre-harvest continuous light delivered by red and blue light-emitting diodes. Journal of Plant Nutrition, 36(3), 481-490.‏
-          White, P. J. & Broadley, M. R. (2003). Calcium in plants. Annals of Botany, 92(4), 487-511.‏
-          White, P. J. (2000). Calcium channels in higher plants. Biochimica et Biophysica Acta Biomembranes, 1465(1), 171-189.‏
-          Wu, M. C., Hou, C. Y., Jiang, C. M., Wang, Y. T., Wang, C. Y., Chen, H. H. & Chang, H. M. (2007). A novel approach of LED light radiation improves the antioxidant activity of pea seedlings. Food Chemistry, 101(4), 1753-1758.‏
-          Yahia, E. M., Contreras-Padilla, M. & Gonzalez-Aguilar, G. (2001). Ascorbic acid content in relation to ascorbic acid oxidase activity and polyamine content in tomato and bell pepper fruits during development, maturation and senescence. Lebensmittel-Wissenschaftund Technologie- Food Science and Technology, 34(7), 452-457.‏
-          Yang, H., Wu, Q., Ng, L. Y. & Wang, S. (2017). Effects of vacuum impregnation with calcium lactate and pectin methylesterase on quality attributes and chelate-soluble pectin morphology of fresh-cut papayas. Food and Bioprocess Technology, 10(5), 901-913.‏
-          Zamaniyan, M., Panahandeh, J., Tabatabaei, S. J. & Motallebie-Azar, A. (2012). Effects of different ratios of K: Ca in nutrient solution on growth, yield and chicon quality of witloof chicory (Cichorium intybus L.). International Journal of Agricultural Science, 2(12), 1137-1142.‏
-          Zhou, Y. & Singh, B. R. (2004). Effect of light on anthocyanin levels in submerged, harvested cranberry fruit. Journal of Biomedicine and Biotechnology, 5, 259-263.‏