عنوان مقاله [English]
Medicinal plants are valuable resources that have been considered by developing and developed countries considered as raw materials to become safe drugs for humans. Molecular DNA barcoding method is a reliable tool for identifying medicinal plants at the genus and species level. In this technique, a small part of the plant genome is sequenced and used to identify and determine the relationship between different species of a genus. In the present study, 7 ecotypes of savory (S. horentiss, S. bakhtiari, S. biossier, S. mutica, S. bakhtiari) were investigated using DNA barcoding. Genomic DNA was extracted by Doyle et al.'s method in the biotechnology laboratory of Ilam University in 1397 and PCR was performed using specific primers for matk, rbcL and ITS genes. After purification of PCR products, it was found that of the studied genes, only matk gene showed good quality for sample separation. Also, nucleotide search results showed that there is a very high similarity (more than 96%) between the obtained sequences and the sequences on the NCBI database. Cluster analysis based on UPGMA method of matk gene showed that the samples of S. biossier and S. bakhtiarica of Ilam and Gilan along with the sample of s. hortensis Birjand and Yazd were in the same group and samples of s. bakhtiari Yazd and s. Khorasan mutica were in the second group.