تأثیر پلی‌آمین‌ها بر شاخص‌های جوانه‌زنی بذر بامیه (Abelmoschus esculentus L.) رقم بسنطی در زمان‌ها و دماهای مختلف

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران

2 دانشجوی دکتری گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران

3 دانشیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

10.22034/iuvs.2020.72727.1019

چکیده

به‌منظور بررسی اثر پلی‌آمین‌ها بر شاخص‌های جوانه‌زنی و رشد گیاهچه بامیه (Abelmoschus esculentus L.) رقم بسنطی در دماهای متفاوت دو آزمایش فاکتوریل در قالب طرح کاملاً تصادفی با چهار تکرار در سال 1393 انجام شد. در آزمایش اول، تیمارهای آزمایشی شامل سطوح مختلف مدت زمان (12 و 24 ساعت) و پیش‌تیمار بذر با غلظت‌های مختلف اسپرمین و اسپرمیدین (0، 5/0، 1، 5/1 و 2 میلی‌مولار) بود. ابتدا زمان بهینه برای پیش‌تیمار بذر بامیه با اسپرمین و اسپرمیدین تعیین شد. سپس بذرهای بامیه با اسپرمین و اسپرمیدین در غلظت‌های ذکر شده در زمان 12 ساعت پیش‌تیمار شدند و آزمون‌های جوانه‌زنی و رشد گیاهچه برای بذرهای پیش‌تیمار شده در دماهای 10، 15، 20 و 25 درجه سانتی‌گراد در آزمایش دوم صورت گرفت. نتایج آزمایش نشان داد که با کاهش دما، شاخص‌های جوانه‌زنی و رشد گیاهچه بامیه کاهش یافت و بهترین دما برای جوانه‌زنی بامیه 25 درجه سانتی‌گراد بود. به‌علاوه پیش‌تیمار بذر بامیه با اسپرمین و اسپرمیدین سبب افزایش معنی‌دار تمامی صفت‌های اندازه‌گیری شده بامیه در دمای 25 درجه سانتی‌گراد شد. کاربرد پیش‌تیمار با غلظت‌های 5/0 و 1 میلی‌مولار اسپرمین و اسپرمیدین برای بهبود شاخص‌های جوانه‌زنی بذر بامیه در دماهای مختلف مطلوب است.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Polyamines on Seed Germination Charactristics of Okra (Abelmoschus esculentus L. Basenti) under Different Time and Temperatures

نویسندگان [English]

  • Behrooz Esmaielpour 1
  • Somayeh Bahadori 2
  • Seror Khoramdel 3
1 Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardebil, Iran
2 Ph.D. Student, Department of Horticulture Sciences, Faculty of Agriculture, University of Mohaghegh Ardebil, Ardebil, Iran
3 Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده [English]

In order to investigate the effects of seed priming by polyamines on germination and seedling growth charecteristic of okra (Abelmoschus esculentus L .Basenti) at different temperatures two laboratory experiments were conducted as factorial layout based on a completely randomized design with four replications in 2014. In the first experiment, treatments were consisted two times (12 and 24 hours) and seed priming with five concentrations of spermine and spermidine (0, 0.5, 1, 1.5 and 2 mM). To determine the optimal time for okra seeds priming. The optimal seeds priming was occurred at 12 hours for all concentrations of spermine and spermidine. There after, seed germination and seedling growth study of primed seeds were carried out at temperatures of 10, 15, 20 and 25 °C in the second. Results showed that both seed germination and seedling growth of okra decreased by decreasing of temperature. The highest seed germination was observed at 25 °C. In addition, seed priming by spermine and spermidine caused significant increase in all germination and seedling growth characteristics of okra at 25 °C. Seed priming at 0.5 and 1 mM concentrations of spermine and spermidine were more efficient to improve seed germination and seedling growth charactristics of okra under different temperatures.

کلیدواژه‌ها [English]

  • Spermidine
  • Spermine
  • Chilling stress
  • Seed Germination
  • Hormone-priming
 

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