Journal of Vegetables Sciences

Journal of Vegetables Sciences

Genetic Diversity Assessment of Some Iranian Parsley (Petroselinum crispum Mill.) Accessions Using Srap Molecular Marker

Document Type : Original Article

Authors
Khadijeh Nasiri 1
Abdolali Shojaeiyan 2
Abbas Yadollahi 2
Amin Mirshekari 3
Khadijeh Ghanbari 4
1 M.Sc. Graduate, Department of Horticultural Sciences, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Iran
2 Assistant Professor, Department of Horticulture Science, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Iran
3 Assistant Professor, Department of Horticulture Science, Faculty of Agriculture, University of Yasuj, Yasuj, Iran
4 M.Sc. Graduate, Department of Horticultural Sciences, Faculty of Agriculture, University of Ilam, Ilam, Iran
10.22034/iuvs.2015.15346
Abstract
This study was performed to evaluate the information and efficiency of SRAP marker for estimating genetic diversity in 15 Iranian parsley stands. Amplification of gene loci was performed using four SRAP primer compounds. The highest and lowest number of polymorphic bands formed were Me2-Em5 and Me4-Em1 primers, respectively. The average number of polymorphic bands per primer combination was 8.25. Cluster analysis of molecular traits based on Jaccard similarity coefficient and Neighbor-Joining algorithm divided the studied populations into five groups at a genetic distance of 0.06. Finally, the results of this study showed that there is a great genetic diversity among the populations collected from several geographical regions of Iran and this study showed the usefulness of SRAP marker in determining the genetic diversity among parsley populations. 
Keywords
Genetic diversity
Parsley
Molecular markers
SRAP indicator
  • Amar, M. H., Biswas, M. K., Zhang, Z. & Guo, W. W. (2011). Exploitation of SSR, SRAP and CAPS-SNP markers for genetic diversity of Citrus germplasm collection. Scientia Horticulturae, 128(3), 220-227.‏
  • Cai, X., Feng, Z., Zhang, X., Xu, W., Hou, B. & Ding, X. (2011). Genetic diversity and population structure of an endangered Orchid (Dendrobium loddigesii Rolfe) from China revealed by SRAP markers. Scientia Horticulturae, 129(4), 877-881.‏
  • Daneshvar, M. H. (2000). Vegetable growing. Shahid Chamran University Press. (In Farsi)
  • Ghanbary, K. (2011). Assessment of genetic diversityamong some Iranian Coriander (Coriander sativum) accession using SRAP markers. M. Sc. Thesis. Faculty of Agricultural Ilam University. (In Farsi)
  • Huang, C. Q., Zhang, Y. F., Liu, G. D., Bai, C. J. & Wang, W. Q. (2012). Genetic diversity of Cynodon radiatus assessed by sequence-related amplified polymorphism markers. Biochemical Systematics and Ecology, 40, 56-61.‏
  • Kumar, L. S. (1999). DNA markers in plant improvement: an overview. Biotechnology advances, 17(2-3), 143-182.‏
  • Li, G. & Quiros, C. F. (2001). Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and applied genetics, 103(2), 455-461.‏
  • Salehi Sormaghi, M. H. (1999). medicinal plants and phytotherapy. World of Nutrition. (In Farsi)
  • Stift, G., Pachner, M. & Lelley, T. (2003). Comparison of RAPD fragment separation in agarose and polyacrylamide gel by studying Cucurbita species. Cucurbit Genet. Coop. Rep, 26, 62-65.‏
  • Wang, S., Yang, W. & Shen, H. (2011). Genetic diversity in Apium graveolens and related species revealed by SRAP and SSR markers. Scientia Horticulturae, 129(1), 1-8.‏
  • Yildiz, M., Ekbic, E., Keles, D., Sensoy, S. & Abak, K. (2011). Use of ISSR, SRAP, and RAPD markers to assess genetic diversity in Turkish melons. Scientia Horticulturae, 130(1), 349-353.‏
  • Zhao, W., Fang, R., Pan, Y., Yang, Y., Chung, J., Chung, I. & Park, Y. (2009). Analysis of genetic relationships of mulberry (Morus L.) germplasm using sequence-related amplified polymorphism (SRAP) markers. African Journal of Biotechnology, 8, 2604-2610.

  • Receive Date 05 December 2015