Journal of Vegetables Sciences

Journal of Vegetables Sciences

Study the Effect of Some Plants Extracts on Growth of Tomato (Solanum lycopersicom L.) in ex-Vitro and in-Vitro Conditions

Document Type : Original Article

Authors
Omid Sohrabi 1
Abdullah Hatamzadeh 2
Azim Ghasemnezhad 3
Habibollah Samizadeh 4
Vahid Erfani Moghadam 5
1 Ph.D. Student of Horticultural Sciences, Faculty of Agriculture Sciences, University of Guilan, Rasht, Iran
2 Professor, Department of Horticultural Sciences, Faculty of Agriculture Sciences, University of Guilan, Rasht, Iran
3 Associate Professor, Department of Horticultural Sciences, Faculty of Plant Productions, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
4 Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture Sciences, University of Guilan, Rasht, Iran
5 Assistant Professor, Department of Nanotechnology, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran
10.22034/iuvs.2022.543372.1184
Abstract
Extended Abstract

Introduction: Today, due to the destructive environmental effects of fertilizers and chemical toxins, the application of plant extracts to improve plant growth and controlling pests and diseases is of particular importance. For assaying the effect of some plant extract on tomato growth behavior, the present study was conducted both as ex-vitro and in-vitro experiments.
Material and Methods: The aim of experiment was to investigate the effect of extract of some plants including Taraxacum officinale, Concharpus erectus, Allium jesdianum, Rheum ribes, Dorema aucheri and Juniperus sabina. Ex-vitro experiment was performed as a completely randomized design with six treatments in three concentrations of 1000, 5000 and 8000 ppm and three replications. In in-vitro study, the experiment was performed with two concentrations of 50 and 100 ppm of plant extracts. The experiment was done at Gorgan University of Agricultural Sciences and Natural Resources in 2021-2022.
Results and Discussion: Results showed that in greenhouse and in most treatments, foliar application of tomato plnts by phytoextracts increased stem length (15 %), internode distance (20 %) and stem diameter. In contrast to that, in some treatments the number of branches (15 %), chlorophyll content and number of flowers (40 %) decreased compared to the control. Under in vitro conditions, germination rate (100 %), seedling growth (37 %), the callus formation (≥ 100 %) and callus weight increased as treated with the plant extracts. The analysis of used plant extracts indicates that they are rich sources of phenolic, flavonoid and antioxidant compounds. High antioxidant activity could be the main reason of observed results. Antioxidant compounds improve cell growth and even improve seed germination via the reducing the stress conditions. However, the role of these compounds in modifying the ratio of endogenous hormones in cultured tissue is not ignorable.
Conclusion: According to the approaches of using plant extracts for nutrition and pest and disease control, the results of the present study in Tomato cultivation is considerable.
Keywords
Callus
Foliar application
Green agriculture
Medicinal plant
Plant for plant
  • Alizadeh, M. (2011). A practical manual for plant tissue culture and micropropgagation. Norozi Publication, Golestan, Iran. (In Persian)
  • Baziramakenga, R., Simard, R. R. & Leroux, G. D. (1994). Effects of benzoic and cinnamic acids on growth, mineral composition, and chlorophyll content of Soybean. Journal of Chemical Ecology, 20(11), 2821-2833.
  • Brand-Williams, W., Cuvelier, M. E. & Berset, C. L. W. T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28(1), 25-30.
  • Chang, C. C., Yang, M. H., Wen, H. M. & Chern, J. C. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3), 1-10.
  • Darier, S. M. & Tammam, A. A. (2012). Potentially phytotoxic effect of aqueous extract of Achillea santolina induced oxidative stress on Vicia faba and Hordeum vulgare. Romanian Journal of Biology-Plant Biology, 57(1), 3-26.
  • De Saeger, J., Van Praet, S., Vereecke, D., Park, J., Jacques, S., Han, T. & Depuydt, S. (2020). Toward the molecular understanding of the action mechanism of Ascophyllum nodosum extracts on plants. Journal of Applied Phycology, 32(1), 573-597.
  • Fornes, F., Sanchez-Perales, M. & Guadiola, J. L. (2002). Effect of a seaweed extract on the productivity of ‘de Nules’ clementine mandarin and navelina orange. Botanica Marina, 45(5), 486-489.
  • Garda, T., Riba, M., Vasas, G., Beyer, D., Marta, M., Hajdu, G. & Mathe, C. (2015). Cytotoxic effects of cylindrospermopsin in mitotic and non-mitotic Vicia fabaChemosphere, 120, 145-153.
  • Gulzar, A., Siddiqui, M. B. & Bi, S. (2016). Phenolic acid allelochemicals induced morphological, ultrastructural, and cytological modification on Cassia sophera and Allium cepa L. Protoplasma, 253(5), 1211-1221.
  • Gulzar, A. & Siddiqui, M. B. (2017). Allelopathic effect of Calotropis procera (Ait.) R. Br. on growth and antioxidant activity of Brassica oleracea botrytis. Journal of the Saudi Society of Agricultural Sciences, 16(4), 375-382.
  • Ishak, M. S. & Sahid, I. (2014). Allelopathic effects of the aqueous extract of the leaf and seed of Leucaena leucocephala on three selected weed species. AIP Conference Proceedings, 1614(1), 659-664.
  • Jose, S. & Gillespie, A. R. (1998). Allelopathy in black Walnut (Juglans nigra) alley cropping. II. Effects of Juglone on hydroponically grown Corn (Zea mays L.) and Soybean (Glycine max L. Merr.) growth and physiology. Plant and Soil, 203(2), 199-206.
  • Kengar, Y. D. & Patil, B. J. (2018). Allelopathic influence of Celosia Argentea against α-amylase activity in Lens Culanaris Medic. during seed germination. International Journal for Science and Advance Research in Technology, 4(1), 420-423.
  • Kocaccedil, I., Ceylan, M. & Terzi, I. (2009). Effects of Juglone on seedling growth in intact and coatless seeds of Cucumber (Cucumis sativus Beith Alpha). Scientific Research and Essays, 4(1), 039-041.
  • Magnitskiy, S. V., Pasian, C. C., Bennett, M. A. & Metzger, J. D. (2006). Controlling plug height of verbena, celosia, and pansy by treating seeds with paclobutrazol. Horticultural Science, 41(1), 158-161.
  • Meda, A., Lamien, C. E., Romito, M., Millogo, J. & Nacoulma, O. G. (2005). Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chemistry, 91(3), 571-577.
  • Mushtaq, W., Siddiqui, M. B. & Hakeem, K. R. (2020). Allelopathy: potential for green agriculture. Springer Nature.
  • Natarajan, A., Elavazhagan, P. & Prabhakaran, J. (2014). Allelopathic potential of billy goat weed Ageratum conyzoides and Cleome viscosa L. on germination and growth of Sesamum indicum L. International Journal of Current Biotechnology, 2(2), 21-24.
  • Naz, R. & Bano, A. (2013). Effects of Calotropis procera and Citrullus colosynthis on germination and seedling growth of Maize. Allelopathy Journal, 31(1), 105-116.
  • Nemati Paykani, M. & Jalilian, N. (2012). Medicinal plants of Kermanshah province. Taxonomy and Biosystematics, 4(11), 69-78. (In Persian)
  • Paradikovic, N., Teklic, T., Zeljkovic, S., Lisjak, M. & Spoljarevic, M. (2019). Biostimulants research in some horticultural plant species-A review. Food and Energy Security, 8(2), e00162.
  • Qasem, J. R. & Foy, C. L. (2001). Weed allelopathy, its ecological impacts and future prospects: a review. Journal of Crop Production, 4(2), 43-119.
  • Rayorath, P., Jithesh, M. N., Farid, A., Khan, W., Palanisamy, R., Hankins, S. D. & Prithiviraj, B. (2008). Rapid bioassays to evaluate the plant growth promoting activity of Ascophyllum nodosum (L.) Le Jol. using a model plant, Arabidopsis thaliana (L.) Heynh. Journal of Applied Phycology, 20(4), 423-429.
  • Reigosa, M. J. & Souto, X. C. (1999). Effect of phenolic compounds on the germination of six weeds species. Plant Growth Regulation, 28(2), 83-88.
  • Rietveld, W. J. (1983). Allelopathic effects of juglone on germination and growth of several herbaceous and woody species. Journal of Chemical Ecology, 9(2), 295-308.
  • Roughani, A. F. R. A., Miri, S. M., Kashi, A. K. & Naserian Khiabani, B. (2017). Increasing the ploidy level in Spinach (Spinacia oleracea) using mitotic inhibitors. Plant Cell Biotechnology and Molecular Biology, 18(4), 124-130.
  • Sanderson, K. C. (1973). Screening chemicals for controlling growth and flowering of Forsythia intermedia zabel. Horticultural Science, 8, 477-492.
  • Sarkar, E., Chatterjee, S. N. & Chakraborty, P. (2012). Allelopathic effect of Cassia tora on seed germination and growth of Mustard. Turkish Journal of Botany, 36(5), 488-494.
  • Sodaeizadeh, H., Rafieiolhossaini, M. & Van Damme, P. (2010). Herbicidal activity of a medicinal plant, Peganum harmala and decomposition dynamics of its phytotoxins in the soil. Industrial Crops and Products, 31(2), 385-394.
  • Suzuki, S., He, Y. & Oyaizu, H. (2003). Indole-3-acetic acid production in Pseudomonas fluorescens HP72 and its association with suppression of creeping bentgrass brown patch. Current Microbiology, 47(2), 0138-0143.
  • Taiz, L., Zeiger, E., Moller, I. M. & Murphy, A. (2015). Plant physiology and development(No. Ed. 6). Sinauer Associates Incorporated.
  • Tan, C. Y., Dodd, I. C., Chen, J. E., Phang, S. M., Chin, C. F., Yow, Y. Y. & Ratnayeke, S. (2021). Regulation of Algal and Cyanobacterial auxin production, physiology, and application in agriculture: an overview. Journal of Applied Phycology, 33(5), 2995-3023.
  • Terzi, I., Kocacaliskan, I., Benlioglu, O. & Solak, K. (2003). Effects of Juglone on growth of Cucumber seedlings with respect to physiological and anatomical parameters. Acta Physiologiae Plantarum, 25(4), 353-356.
  • Vernieri, P., Borghesi, E., Ferrante, A. & Magnani, G. (2005). Application of biostimulants in floating system for improving rocket quality. Journal of Food Agriculture and Environment, 3(3), 86-91.
  • Volfova, A., Chvojka, L. & Friedrich, A. (1978). The effect of kinetin and auxin on the chloroplast structure and chlorophyll content in Wheat coleoptiles. Biologia Plantarum, 20(6), 440-445.
  • Wu, H., Pratley, J., Lemerle, D. & Haig, T. (2001). Allelopathy in Wheat (Triticum aestivum). Annals of Applied Biology, 139(1), 1-9.
  • Zou, L., Pan, C., Wang, M. X., Cui, L. & Han, B. Y. (2020). Progress on the mechanism of hormones regulating plant flower formation. Yi Chuan Hereditas, 42(8), 739-751.
Journal of Vegetables Sciences
Volume 5, Issue 10 - Serial Number 2
January 2023
Pages 181-197

  • Receive Date 21 November 2021
  • Revise Date 07 February 2022
  • Accept Date 09 February 2022