Evaluation of Glycine Amino Acid Application on Growth, Morphophysiological and Biochemical Characteristics of Cucumber under Deficit Irrigation Levels

Zargar SHOSHTARI, Fatemeh; Souri, Mohammad Kazem; Hasandokht, Mohammadreza; Kalateh Jari, Sepideh

doi:10.22034/iuvs.2022.553061.1203

Evaluation of Glycine Amino Acid Application on Growth, Morphophysiological and Biochemical Characteristics of Cucumber under Deficit Irrigation Levels

Document Type : Original Article

Authors

Fatemeh Zargar SHOSHTARI ¹

mohammad Kazem Souri ²

Mohammadreza Hasandokht ³

Sepideh Kalateh Jari ⁴

¹ Department of Horticultural Science, Faculty of Agriculture, Sciences and Researches Branch, Islamic Azad University, Tehran, Iran

² Department of Horticultural Science, Faculty of Crop Sciences Sari Agricultural Sciences and Natural Resources Unicersity, Sari, Iran

³ Department of Horticultural Science, Faculty of Agriculture, University of Tehran , Alborz, Iran

⁴ Department of Horticultural Science, Faculty of Agriculture, Science and Research Branch, Islamic Azad University, Tehran, Iran

10.22034/iuvs.2022.553061.1203

Abstract

1.Introduction: Water shortage is an integral part of our current agriculture, and water stress is one of the most destructive environmental factors which adversely affects agricultural production worldwide. According to several studies, water deficit causes reduction of vegetative growth, leaf area, plant height, dry weight, photosynthesis rate, chlorophyll content, stomatal conductance, enzymatic activities, protein biosynthesis and amino acids accumulation in horticultural plants. Increasing reactive oxygen species (ROS) rate and activity is the one of most damaging effects of drought stress. Cucumber is a tropical crop with high water requirement. Wide leaves and shallow roots have made it a drought-sensitive crop. Based on reports, water deficit and drought stress can seriously affect yield performance and qualitative characteristics of cucumber. Measurements are needed in different aspects to overcome challenges associated with water deficit and water stress in cropping systems. So, in this study glycine amino acid were applied for its possible role under mild water shortage effects on cucumber growth characteristics.
2.Materials and Methods: In order to investigate the influence of glycine amino acid application on alleviation of negative effects of deficit irrigation, a greenhouse experiment was conducted based on completely randomized design with four replications. Treatments were assessed at six levels including 60 % FC, 70 % FC, 80 % FC, 60 % FC + glycine 500 mg plant-1, 70 % FC + glycine 500 mg plant-1 and 80 % FC + glycine 500 mg plant-1. Some of most important morphophysiological and biochemical characteristics of cucumber plants such as stem length, stem dry weight, leaf number, leaf area, internode length, growth period duration, fruit number, fruit set, vitamin C, chlorophyll index, total antioxidant, proline, catalase, peroxidase and polyphenol oxidase enzymes content were evaluated.
3.Results and Discussion: Result showed that deficit irrigation and glycine amino acid application significantly influenced morphophysiological and biochemical characteristics of cucumber plant. It was observed that vegetative traits including leaf number, leaf area index, stem length and dry weight, length of internode, growth period duration, chlorophyll index, and fruit characteristics including vitamin C content and fruit set were significantly reduced by deficit irrigation levels particularly at 60 % FC. The highest fruit number (86.5) was recorded under 70 % FC and application of glycine. In contrast, antioxidant capacity, proline, catalase, polyphenol oxidase and peroxidase enzymes content increased under 60 % FC. Glycine application could properly alleviate the negative effects of deficit irrigation on plant growth and yield. Studies have shown that glycine application maintains chlorophyll content and photosynthesis rate of plants under stress condition, which is mostly related to increasing stomatal conductance and carboxylation efficiency. Generally, water deficit reduces the yield performance of crops. And, this reduction could be correlated with decreasing leaf area and disruption of nutrients absorption. According to results of this study, maintaining the photosynthetic pigments caused by glycine application resulted in enhanced photosynthesis rate leading to higher yield comparing to control under deficit irrigation conditions.
4.Conclusion: Generally, the results of the current study demonstrated that deficit irrigation at level of 60 % FC caused induction of drought stress conditions. Induction of water deficit and drought stress higher than 70 % FC led to sever physiological and biochemical changes in cucumber plants resulting in considerable reduction of yield performance and qualitative characteristics. Water deficit increased proline content and antioxidant capacity (increasing the activity rate of antioxidant enzymes including catalase and peroxidase) of cucumber plants. Soil application of glycine amino acid alleviated the adverse effects of deficit irrigation. Because the highest fruit number was counted in 70 % FC + glycine 500 mg plant-1 treatment (with no significant difference with the control (80 % FC)), this treatment could be recommended to growers to save water consumption with no significant changes in yield performance comparing to control level.

Keywords

Antioxidant activity

Cucumber

Drought stress

Proline

Vegetables

Vitamin C

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