Journal of Vegetables Sciences

Journal of Vegetables Sciences

The effects of foliar application of sugar beet molasses on the growth and yield of greenhouse tomato (Solanum lycopersicum L. cv. Dafnis)

Document Type : Original Article

Authors
1 Former MSc. Student, Department of Horticultural Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2 Professor, Department of Horticultural Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 Associate Professor, Department of Horticultural Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
4 Laboratory Expert, Department of Horticultural Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Abstract
Extended Abstract
1. Introduction: Due to the increase in tomato cultivation in greenhouses, the need for organic nutrition to increase the yield and health of the product is more necessary than ever. Sucrose is the main transportable product of photosynthesis in the phloem vessels, which is absorbed through the leaf, and especially in low light conditions, it provides the necessary energy for the growth of reproductive organs and increases the growth of the fruit. The use of natural compounds, such as beet molasses, in combination with sucrose can have positive effects on the quantitative and qualitative growth of tomatoes. Despite the studies of sucrose in the form of foliar spraying in plants, there is little information about the effect of sugar beet molasses in greenhouse conditions. Therefore, in this research, the effects of foliar application of sugar beet molasses on the quantitative and qualitative characteristics of greenhouse tomatoes of the Dafnis variety were studied.
2. Materials and methods: The treatments included sugar beet molasses in concentrations of 0, 5, 10, and 15%, which were applied as a foliar spray one week after plant establishment and repeated once every 10 days. The experiment was conducted in the form of a randomized complete block design with four treatments and three replications. The measured variables included plant height, leaf length, leaf width, fruit firmness, soluble solids, acidity, vitamin C, total sugar, glucose, sucrose, fructose, lycopene, chlorophyll, carotenoid, anthocyanin, total nitrogen, phosphorus, potassium, calcium, and magnesium. Plant height measurement and yield were calculated based on plant height and fruit weight up to the first three clusters of the plant. The test data was analyzed using SAS statistical software. The mean comparisons for each attribute were also done using Duncan's test at the probability level of 5%.
3. Results and Discussion: The results showed that the highest plant height (55.43 cm), fruit firmness (4.23 kgcm2-1), chlorophyll a (1.4 mgg-1 FW), chlorophyll b (0.423 mgg-1 FW), total chlorophyll (1.824 mgg-1 FW), and anthocyanin (0.216 µg-1 FW) were related to the 10% sugar beet molasses. The highest fruit yield (1600 g per plant) was related to the 5% molasses, which was statistically not significantly different from the 10% molasses. The findings of phytochemical compounds revealed that the highest amount of soluble solids (5.03%), vitamin C (2.38 mg/100 ml of fruit juice), and lycopene (122.11 mgg-1) was found in 10% molasses sugar beet has been theitized. Also, the highest amount of total sugar (26.13 mgg-1), glucose (7.36 mgg-1), sucrose (4.12 mgg-1), and fructose (1.92 mg/g) was observed in 10 % of molasses. The highest amount of total phenol (15.95 mgg-1), flavonoid (2.71 mgg-1), and antioxidant (61.48%) was related to the 10% molasses. The highest amount of nitrogen (3.8%), phosphorus (0.328%), potassium (4.04%), calcium (2.62 mgg-1), and magnesium (2.14 mgg-1) was obtained in foliar application of 10% molasses. Tomato is a light-demanding plant, so light is the limiting factor in its greenhouse production. During the winter months, due to the short day length and low radiation intensity, the amount of light received during the day is greatly reduced, which causes the reduction of greenhouse tomato yield. Sugar compounds can have a positive effect on the increase of photosynthetic pigments. Therefore, foliar application of molasses can improve the chlorophyll concentration, and at the same time, it does not cause photosynthetic inhibition in low light conditions of the greenhouse. External foliar spraying of beet molasses causes foliar absorption of sugar and can help tomato plants as an effective method, especially in conditions of low photosynthetic production. Soluble sugars, especially sucrose, glucose, and fructose, play an important role in plant structure and metabolism.
4. Conclusion: In general, the results of this research showed that the amount of photosynthetic pigments, anthocyanins, soluble sugars, antioxidant compounds, and macro elements in 10% sugar beet molasses was significantly higher than the control. The 5% molasses increased the yield and finally the weight of the tomato fruit. The role of sugar beet molasses becomes effective when the plant is exposed to the stress of a lack of light (such as greenhouse conditions in the winter months), because photosynthetic production is limited in the stress of light deficiency. Future research should be focused on sugar transporter minerals such as potassium nitrate and magnesium nitrate. In this experiment, evidence was presented that sugar beet molasses changes the biochemical reaction of tomato to increase fruit quality and total yield.
Keywords

Aljabri, M., Alharbi, S., Al-Qthanin, R.N., Ismaeil, F.M., Chen, J. & Abou-Elwafa, S.F. (2021). Recycling of beet sugar byproducts and wastes enhances sugar beet productivity and salt redistribution in saline soils. Environmental Science and Pollution Research, 28, 45745–45755.https://doi.org/10.1007/s11356-021-13860-3
AOAC. (1984). Official Methods of Analysis. Association of official Analytial chemists. Washington, DC., U.S.A. 114p.
Azarakhsh, M. R., Bagherieh-Najjar, M. B., Sadeghipour, H. R., & Raeisi, S. (2022). Improved grain yield by phytohormones-driven suppression of pod abscission and revitalization of source-sink relationships in soybean. International Journal of Plant Production, 16, 3, 467-481. (In Persian) https://doi.org/10.1007/s42106-022-00196-2
Cao, Y.Y., Yanga, M.T., Lia, X., Zhoua, Z.Q., Wanga, X.J. & Baia, J.G. (2014). Exogenous sucrose increases chilling tolerance in cucumber seedlings by modulating antioxidant enzyme activity and regulating proline and soluble sugar contents. Scientia Horticulture, 179, 67-77. https://doi.org/10.1016/j. scienta. 2014.09.016
Cho, L.-H., Pasriga, R., Yoon, J., Jeon, J.-S., & An, G. (2018). Roles of sugars in controlling flowering time. Journal of Plant Biology, 61, 121-130. https://doi.org/10.1007/ s12374- 018-0081-z
Ebrahimzadeh, M. A., Nabavi, S. F., Nabavi, S. M. & Eslami, B. (2010). Antihemolytic and antioxidant activities of Allium paradoxum. Central European Journal of Biology, 5(3), 338-345.‏ https://doi.org/10.2478/s11535-010-0013-5
Farhat, N., Rabhi, M., Krol, M., Barhoumi, Z., Ivanov, A.G., McCarthy, A., Abdelly, C., Smaoui, A., & Huner, N.P.A. (2014). Starch and sugar accumulation in Sulla carnosa leaves upon Mg2+ starvation. Acta Physiology Plant, 36, 2157-2165. https://doi.org/10.1007/s11738-014-1592-y
Ghorbani, D. A., Mashayekhi, K. & Kamkar, B. (2015). Effect of foliar application sucrose, boron, potassium nitrate and salicylic acid on yield and yield components of tomato var. Super A. Research in Crop Ecosystems, 2(1), 43-52. (In Persian)
Handel, E.V. (1968). Direct micro determination of sucrose. Journal Analytic biochemistry, 22, 280-283. https://doi.org/10.1016/0003-2697(68)90317-5
Hatano, K. & Yamatsu, T. (2018). Molasses melanoidin-like products enhance phytoextraction of lead through three Brassica species. International Journal of Phytorem, 20, 552–559. doi: 10.1080/15226514.2017.1393397.
Javanmardi, J., & Emami, S. (2013). Application of sucrose on tomato seedlings improves transplant quality, crop establishment, cold and dark hardiness. Advances in Horticultural Science, 27(3), 122-126.
Kiokiasm, S., Proestosm C., & Oreopouloum V. (2020). Phenolic acids of plant origin- a review on their antioxidant activity in vitro (O/W Emulsion Systems) along with their in vivo health biochemical properties. Foods, 9(4), 534. https://doi.org/10.3390/foods9040534
Kozmińska, A., Hanus-Fajerska, E., Halecki, W., & Ciarkowska, K. (2021). Beet molasses enhances salinity tolerance in Thymus serpyllum—a study under greenhouse conditions. Plants, 10(9),1819. https://doi.org/10.3390/plants10091819
Li, S., Zhao, X., Ye, X., Zhang, L., Shi, L., Xu, F. & Ding, G. (2020). The effects of condensed molasses soluble on the growth and development of rapeseed through seed germination, hydroponics and field trials. Agriculture, 10, 260. https://doi.org/10.3390/agriculture10070260
Marschner, H. (1995). Mineral nutrition of higher plants. Second edition, Academic pressinc, London, 891p.
Mashayekhi, K. & Atashi, S. (2014). The analyzing methods in plant physiology (surveys before and after harvest). Press Sirang Words, Gorgan, 310 p. (In Persian)
mashayekhi, K., & atashi, s. (2012). effect of foliar application of boron and sucrose on biochemical parameters of “camarosa” strawberry. journal of Plant Production, 19(4), 157-171. (In Persian) https://doi.org/20.1001.1.232220501391.19.4.9.1
Mashayekhi, K., & A. Shomali. (2018). Botany, physiology and culture of vegetable. Gorgan University of Agricultural Sciences & Natural Resources press. pp 502. (In Persian)
Mashayekhi, K., Keykha, Z., Movahedi Naeini, S. A., Kamkar, B., & Mousavizadeh, S.J. (2016). Seedling and fruit quality of tomato (Solanum lycopersicum Var. Super A) in response to spraying sucrose and boric acid. Journal of Vegetables Sciences, 2(2), 61-73. https://doi.org/10.22034/iuvs.2016.32819
Mashayekhi, K., Kiani, Z., Mousavizadeh, S.J., & Rostaghi, M. (2023). Greenhouse, physiology of cultivated plants. Gorgan University of Agricultural Sciences & Natural Resources press. pp 350. (In Persian)
McCready, R.M., Guggolz, J., Silviera, V., & Owens, H.S. (1950). Determination of search and amylase in vegetables. Analytical Chemistery, 22, 1156-1158. https://doi.org/10.1021/ac60045a016
McDonald, S., Prenzler, P. D., Antolovich, M. & Robards, K. (2001). Phenolic content and antioxidant activity of olive extracts. Food Chemistry, 73(1), 73-84. https://doi.org/10.1016/S0308-8146(00)00288-0
Miller, G.L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Journal Analytic Chemistry, 31, 426-428. https://doi.org/10.1021/ ac60147a030
Mosanaii, M., Mashayekhi, K., Mehrabanjoubani, P., & Mousavizadeh, S.J.  (2021). Effect of sucrose application and its interaction with boric acid on yield of tomato under greenhouse conditions. 12th Congress of Horticultural Sciences of Iran, (In Persian)
Palmonari, A., Cavallini, D., Sniffen, C.J., Fernandes, L., Holder, P., Fagioli, L., Fusaro, I., Biagi, G., Formigoni, A., & Mammi, L. (2020). Short communication: Characterization of molasses chemical composition. Journal of Dairy Science, 103 (7), 6244-6249. https://doi.org/10.3168/jds.2019-17644

Ribeiro, R.V., Machado, E.C., Filho, J.R.M., Lobo, A.K.M., Martins, M.O., Silveria, J.A.G., Yin, X. & Struik, P.C. (2017). Increased sink strength offsets the inhibitory effect of sucrose on sugarcane photosynthesis.  Journal of Plant Physiology, 208, 61-69. https://doi.org/10.1016/j.jplph.2016.11.005

Salehi, M.R., NizamDoust Darestani, D., & Ghasemi, K. (2021). The effect of foliar spraying of sucrose and some nutrients on the distribution of carbohydrates in radish (Rhaphunus sativus var. sativus). Journal of Horticultural Plant Nutrition, 4,1, 79-96. https://doi.org/10.22070/hpn.2020.5714.1109
Salehi, M.R., & Ghasemi, K. (2022). Effect of foliar application of sucrose and certain mineral elements on flower abortion, carbohydrate partitioning and quality of Capsicum annuum L.. Journal of Vegetables Sciences, 5(2), 19-34. https://doi.org/10.22034/iuvs.2021.534620.1172
Salon, C., Munier-Jolain, N. G., Duc, G., Voisin, A.S., Grandgirard, D., Larmure, A., Emery, R., & Ney, B. (2001). Grain legume seed filling in relation to nitrogen acquisition: a review and prospects with particular reference to pea. Agronomie, 21(6-7), 539-552. https://doi.org/10.1051/agro:2001143
Saric, L., Filipcev, B., Simurina, O., Plavsic, D.V., Saric, B., Lazarevic, J. & Milovanovic, I. (2016). Sugar beet molasses: Properties and applications in osmotic dehydration of fruits and vegetables. Food Feed Research, 43, 135–144. https://doi.org/10.5937/FFR1602135S
Singh, R., Yavdav, M., Kumar, V., Singh, M., & Upadhyay, S.K. (2021). Effect of molasses on the growth of okra Abelmoschus esculentus (L.) Moench (Dicotylenoae: Malvaceae). Bio-Science Research Bulletin, 37, 4–11.
Smolen, S., & Sandy, W. (2009). The effect of foliar nutrition with urea, molybdenum, sucrose and benzyladenine on quantity and quality of radish yield. Hortorum Cultus, 8, 45-55. https://doi.org/10.2478/v10032-010-0009-8
Sousaraei, N., Mashayekhi, K., Mousavizadeh, S.J., Akbarpour, V., Medina, J., & S. Aliniaeifard. (2021). Screening of tomato landraces for drought tolerance based on growth and chlorophyll fluorescence analyses. Horticulture, Environment and Biotechnology, 62, 521-535. https://doi.org/10.1007/s13580-020-00328-5
Sousaraei, N., Mashayekhi, K., Mousavizadeh, S.J., & Dadrasi, A. (2020). Evaluation of morpho-physiological fruit traits of some tomato populations in iran using correlation coefficients and cluster analysis. Journal of Horticultural Science and Technology, 21(1), 61-74.
Srivastava, P.C., Singh, R.K., Srivastava, P., & Shrivastava, M. (2012). Utilization of molasses based distillery effluent for fertigation of sugarcane. Biodegradation, 23, 897–905. https://doi.org/10.1007/s10532-012-9582-5
Taiz, L. & Zeiger, E. (2010). Plant Physiology, Sinauer Associates; 5 edition, 782 p.
Trankner, M., Tavakol, R., & Jakli, B. (2018). Functioning of potassium and magnesium in photosynthesis, photosynthate translocation, and photoprotection. Physiologia Plantarum, 163, 414-431. https://doi.org/10.1111/ppl.12747
Van Le, Q., Samson, G., & Desjardins, Y. (2001). Opposite effects of exogenous sucrose on growth, photosynthesis and carbon metabolism of in vitro plantlets of tomato grown under two levels of irradiances and CO2 concentration. Journal of Plant Physiology, 158, 599-605. https://doi.org/10.1078/ 0176-1617-00315
Walker, G.E. (2007). Effects of organic amendments, fertilizers and feanamiphos on parasitic and free-living nematodes, tomato growth and yield. Nematologia Mediterranea, 35, 131–136.
Wanger, G.J. (1979). Content and vacuole extra vacuole distribution of neutral sugars, free amino acids, and anthocyanins in protoplasts. Plant Physiology, 64, 88-93. https://doi.org/10.1104/pp.64.1.88
Yisa, J. (2009). Phytochemical analysis and antimicrobial activity of Scoparia dulcis and Nymphaea lotus. Australian Journal of Basic and Applied Sciences, 3(4), 3975-3979.
Yoon, H. S., Cho, C. H., Yun, M. S., Jang, S. J., You, H. J., Kim, J. H., & Ko, G. (2021). Akkermansia muciniphila secretes a glucagon-like peptide-1-inducing protein that improves glucose homeostasis and ameliorates metabolic disease in mice. Nature Microbiology, 6(5), 563-573. https://doi.org/10.1038/ s41564-021-00880-5
Volume 9, Issue 17
July 2025
Pages 223-242

  • Receive Date 29 October 2023
  • Revise Date 13 November 2023
  • Accept Date 16 November 2023