Effect of Selenium and Humic Acid on Germination and Some Morphological Characteristics of Quinoa under Drought and Salinity Stress

Amerian, Masoomeh; Khoramivafa, Mahmud; Rabani, Barakat Alah

doi:10.22034/iuvs.2022.537591.1177

Effect of Selenium and Humic Acid on Germination and Some Morphological Characteristics of Quinoa under Drought and Salinity Stress

Document Type : Original Article

Authors

masoomeh amerian ¹

Mahmud Khoramivafa ²

Barakat Alah Rabani ³

¹ Assistant Professor, Department of Production Engineering and Plant Genetics, Faculty of Science and Agriculture Engineering, Razi University, Kermanshah, Iran

² Associate Professor, Department of Production Engineering and Plant Genetics, Faculty of Science and Agriculture Engineering, Razi University, Kermanshah, Iran

³ Department of Production Engineering and Plant Genetics, Faculty of Science and Agriculture Engineering, Razi University, Kermanshah, Iran

10.22034/iuvs.2022.537591.1177

Abstract

Extended Abstract
1. Introduction: In recent years, young quinoa leaves are used as fresh or cooked vegetables. Due to its high quality vegetable protein, quinoa is a promising candidate for increasing food production in the world. Due to its tolerance to abiotic stresses, quinoa is known as one of the most valuable and nutritious food products. Environmental stresses, especially drought and salinity are one of the most important factors in reducing growth in plant growth and development stages, especially the germination stage of the plant. The increase in global demand for the production of nutritious and healthy food has challenged researchers to look for the production of products in marginal areas that are inefficient for producing agricultural products due to adverse weather conditions, low soil fertility, and lack of good quality irrigation water. As a result, in this study was conducted to study the effect of selenium and humic acid on germination percentage, seed germination characteristics and some morphological characteristics of quinoa (Chenopodium quinoa) seedling under drought and salinity stress as a factorial experiment in the form of completely randomized design with three replications.
2. Materials and Methods: The research was conducted as two independent experiments. The experiments were carried out in the physiology laboratory of the Faculty of Agricultural Sciences and Engineering. The first experiment consisted of four levels of selenium (0, 5, 10 and 20 mg L-1 sodium selenate), three levels of humic acid (0, 100 and 300 mg L-1) and four levels of drought stress (0, -4, -8 and -12 bar). The second experiment with four levels of selenium (0, 5, 10 and 20 mg L-1 sodium selenate), three levels of humic acid (0, 100 and 300 mg L-1) and six levels of salinity stress (0, 0.5, 4.3, 8, 11.8 and 16 ds/m) were performed. Titicaca variety was used in this research. The criterion for seed germination was the emergence of roots approximately 3 mm long. At the end of the seventh day, the characteristics of germination percentage, germination rate, mean daily germination, mean germination time, germination rate coefficient, seed vigour and seed germination index were measured. At the end of the experiment, the length, fresh and dry weight of seedlings, stems and roots were examined.
3. Results and Discussion: Drought and salinity stress had a negative effect on germination percentage and seed germination indices and morphological characteristics of quinoa seedlings. Whiles, according to the results, selenium and humic acid under salinity and drought stress had a positive effect on germination percentage and some morphological characteristics of quinoa seedling. Considering that one of the salinity resistance indicators is having longer root length, selenium and humic acid increased the root length of quinoa under salt stress conditions compared to the control. Humic acid and selenium had a positive effect on germination and plant growth by increasing water uptake and root volume, so that the highest germination percentage and morphological characteristics in the treatment without stress with 20 mg L-1 sodium selenate and 300 mg L-1Humic acid was observed. According to the obtained results, the highest percentage of germination, germination rate germination rate coefficient, seed vigor, seedling, stem and root fresh weight in the treatment without salt stress with 20 mg L-1 of sodium selenate and 300 mg L-1 humic acid was observed. With the increase of sodium chloride concentration, the germination percentage and germination indices of quinoa seeds decreased. Under salinity stress, in all four levels of selenium, with increasing humic acid concentration, the percentage of germination and investigated characteristics increased.
4. Conclusion: Therefore, the use of humic acid at a concentration of 300 mg L-1 along with 20 mg L-1 sodium selenate is recommended to improve germination and seed growth of quinoa under drought and salt stress conditions. As a result, the use of selenium and humic acid in the direction of sustainable agriculture and healthy crop production is cost effective and the use of selenium and humic acid fertilizer is recommended. Humic acid improves product performance by increasing nutrient absorption and affecting antioxidant defense mechanisms. In salinity stress, humic acid leads to osmotic regulation by maintaining water absorption. Selenium has beneficial effects on plants at low levels. Selenium can affect germination and root length. Plant seeds are able to absorb selenium from the environment and convert it into various organic forms or inorganic selenium compounds during germination.

Keywords

Ermination percentage

Sodium selenate

Seed vigor

Seedling growth

- Alhariri, A. & Boras, M. (2020). Responses of seed germination and yield related traits to seed pretreatment and foliar spray of humic and amino acids compounds in carrot (Daucus carota L.). International Journal of Chemical Studies, 8(4), 26-30.

- Aliloo, A. A., Shiriazar, Z., Dashti, Sh., Shahabivand, S. & Pourmohammad, A. R. (2020). Alleviating effects of humic acid on germination and vegetative growth of canola under salinity stress. Journal of Plant Research is the Iranian Journal of Biology, 33(4), 985-997.

- Alizadeh Ahmadabadi, A. & Khorasaninejad, S. (2017). The Effect of humic acid pretreatment on germination of purple cornflower (Echinacea purpurea) plant under drought and salinity conditions. Arid Biome Scientific and Research Journal, 6(2), 97-107.

- Amerian, M. & Nosratti, E. (2021). Effect of different levels of nano-selenium and selenium on seed germination and seedling growth of onion (Allium cepa L.). Journal of Vegetables Sciences, 4(8), 67-80. (In Persian)

- Anirudh, K. V. S., Chakraborty, T., Srivastava, R. K. & Akhtar, N. (2020). Effect of drought and salt stress on cereal crop plants and their proteomic and physiological studies. Journal of Biotechnology and Biomedical Science, 2(3), 43-59.

- Aroiee, H., Shekari, L. & Mirshekari, A. (2019). Effects of selenium on damage of heavy metals in germination, growth and antioxidant activities ofcucumber (Cucumis sativus L.) seedling. Iranian Journal of Seed Science and Research, 6(2), 269-286. (In Persian)

- Arslan, E., Agar, G. & Aydin, M. (2021). Humic acid as a biostimulant in improving drought tolerance in wheat: The expression patterns of drought-related genes. Plant Molecular Biology Reporter, ttps://doi.org/10.1007/s11105-020-01266-3

- Ashour, H. A., Esmail, S. E. A. & Kotb, M. S. (2021). Alleviative effects of chitosan or humic acid on Vitex trifolia ‘Purpurea’ grown under salinity stress. Ornamental Horticulture, 27(1), 88-102.

- Azad, H., Fazeli nasab, B. & Sobhanizade, A. A. (2017). Study into the Eeffect of jasmonic and humic acids on some germination characteristics of rosselle (Hibiscus sabdariffa) seed under salinity stress. Iranian Journal of Seed Research, 4(1), 1-18. (In Persian)

- Bijanzadeh, E., Emam, Y. & Pessarakli, M. (2021). Biochemical responses of water-stressed triticale (X Triticosecale wittmack) to humic acid and jasmonic acid. Journal of Plant Nutrition, 44(2), 252-269.

- Borbély, P., Molnár, A., Valyon, E., Ördög, A., Horváth-Boros, K., Csupor, D., Fehér, A. & Kolbert, Z. (2021). The effect of foliar selenium (Se) treatment on growth, photosynthesis, and oxidative-nitrosative signalling of stevia rebaudiana leaves. Antioxidants, 10(1), 1-18.

- ElFayomy, A. H., El-Behairy, U. A. A. & Mona, M. Y. (2021). Response of lettuce to humic acid and salt stress condition under soilless culture system. Journal of the Advances in Agricultural Researches, 26(1), 20-38.

- Gholami, Sh., Amini Dehaghi, M. & Rezazadeh, A. R. (2022). Effect of different concentrations of selenium on germination characteristics and proline content of quinoa (Chenopodium quinoa willd) under drought stress. Environmental Stresses in Crop Sciences, 14(4), 1029-1040.

- Guardado-Félix, D., Serna-Saldivar, S.O., Gutiérrez-Uribe, J. A. & Chuck-Hernández, C. (2019). Selenium in germinated chickpea (Cicer arietinum L.) increases the stability of its oil fraction. Plants (Basel), 8)113(, 1-10.

- Hernández-Hernández, H., Quiterio-Gutiérrez, T., Cadenas-Pliego, T., Ortega-Ortiz, H., Hernández-Fuentes, A. L., de la Fuente, M. C., Valdés-Reyna, J. & Juárez-Maldonado, A. (2019). Impact of selenium and copper nanoparticles on yield, antioxidant system, and fruit quality of tomato plants. Plants, 8(10), 1-17.

- Hu, E., Liu, M., Zhou, R., Jiang, F., Sun, M., Wen, J., Zhu, Zh. & Wua, Zh. (2021). Relationship between melatonin and abscisic acid in response to salt stress of tomato. Scientia Horticulturae, 285(1), 110176.

- Huihui, Z., Yuze, H., Kaiwen, H., Zisong, X., Liu, Sh., Wang, Q., Wang, X., Nan, X., Wu, Y. & Guangyu, S. (2021). Na+ accumulation alleviates drought stress induced photosynthesis inhibition of PSII and PSI in leaves of Medicago sativa. Journal of Plant Interactions, 16(1), 1-11.

- Hussein, S. A., Noori, A. M. & Kanber, H. S. (2020). Stratification period with different agricultural media roll on seeds germination ratio and humic acid fertilization on apricot seedlings Prunus armeniaca L. Growth‏. Plant Cell Biotechnology and Molecular Biology, 21(71&72), 23-29.

- Koskosidis, A., Ebrahim Khah, E., Mavromatis, A., Pavli, O. & Vlachostergios, D. N. (2020). Effect of PEG-induced drought stress on germination of ten chickpeas (Cicer arietinum L.) genotypes. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(1), 294-304.

- Lapaz, A. M., Santos, L. F.M., Yoshida, C. H. P., Heinrichs, R., Campos, M. & Reis, A. R. (2019). Physiological and toxic effects of selenium on seed germination of cowpea seedlings. Bragantia, 78(4), 498-508.

- Lei, Y., Liu, Q., Hettenhausen, C., Cao, G., Tan, Q., Zhao, W., Lin, H. & Wu, J. (2017). Salt-tolerant and –sensitive alfalfa (Medicago sativa) cultivars have large variations in defense responses to the lepidopteran insect Spodoptera litura under normal and salt stress condition. PLoS ONE, 12(7), 1-15.

- Liang, Y., Li, D., Chen, Y., Cheng, J., Zhao, G., Fahima, T. & Yan, J. (2020). Selenium mitigates salt-induced oxidative stress in durum wheat (Triticum durum Desf.) seedlings by modulating chlorophyll fluorescence, osmolyte accumulation, and antioxidant system. Biotech, 10(8), 368.

- Lu, N., Wu, L. & Shi, M. (2020). Selenium enhances the vase life of Lilium longiflorum cut flower by regulating postharvest physiological characteristics. Scientia Horticulturae, 264, 109172.

- Man-hong, Y., Lei, Z., Sheng-tao, X., McLaughlin, N. B. & Jing-hui, L. (2020). Acid applied to potato foliage on plant growth, photosynthesis characteristics and fresh tuber yield under different water deficits. Scientific reports, 10(1), 1-10

- Mazhar, T., Ali, Q., Rashid, M. S. & Mailk, A. (2020). Effects of salt and drought stress on growth raits of Zea mays seedlings. Life Science Journal, 17(7), 48-54.

- Mona Nossier, I., Gawish, Sh. M., Taha, T. A. & Mubarak, M. (2017). Response of wheat plants to application of selenium and humic acid under salt stress conditions. Egyptian Journal of Soil Science, 57(2), 175-187.

- Mardi, M., Abbasifar, A.R & ValizadehKaji, B. 2022. Comparison of the effect of bological and non-biological fertilizers on quantitative, qualitative and phytochemical properties of garlic (Allium sativum L.). Journal of Vegetables Science, 10(2): 1-17. (In Persian)

- Mosavi, S. A. & Aghighi Shahverdi, M. (2021). Effect of seeding priming with selenium on germination and physiological characteristics of dragons head (Lallemantia iberica) under salinity stress. Journal of Seed Research, 10(2), 65-76.

- Mousavi, S. E. & Aghighi Shahverdi, M. (2021). Effect of nutritious seed priming with selenium on germination and physiological indices of Dragon head (Lamellia ibrica L.) under salinity stress. Journal of Seed Research, 10(35), 65-76. (In Persian)

- Nawaz, F., Zulfiqar, B., Ahmad, K. S., Majeed, S., Shehzad, M. A., Javeed, H. M. R., Tahir, N. N. & Ahsan, M. (2021). Pretreatment with selenium and zinc modulates physiological indices and antioxidant machinery to improve drought tolerance in maize (Zea mays L.). South African Journal of Botany, 138(1), 209-216.

- Purwanto, B. H., Wulandari, P., Sulistyaningsih, E., Utami, S. N. H. & Handayani, S. (2021). Department of soil science, improved corn yields when humic acid extracted from composted manure is applied to acid soils with phosphorus fertilizer. Applied and Environmental Soil Science, 1-12.

- Qureshi, A. S. & Daba, A. W. (2020). Evaluating growth and yield parameters of five quinoa (Chenopodium quinoa) genotypes under different salt stress conditions. Journal of Agricultural Science, 12(3), 128-140.

- Rajabi Dehnavi, A., Zahedi, M., Ludwiczak, A., Cardenas Perez, S. & Piernik, A. (2020). Efect of salinity on seed germination and seedling development of sorghum (Sorghum bicolor L.) Moench genotypes. Agronomy, 10(6), 1-15.

- Ren, Y., Wang, W., He, J., Zhang, L., We, Y. & Yang, M. (2020). Nitric oxide alleviates salt stress in seed germination and early seedling growth of pakchoi (Brassica chinensis L.) by enhancing physiological and biochemical parameters. Ecotoxicology and Environmental Safety, 187(1), 1-10.

- Roman, V. J., den Tooma, L. A., Gamiza, C. C., van der Pijl, N., Vissera, R. G., van Loo, E. N. & van der Linden, G. G. (2020). Differential responses to salt stress in ion dynamics, growth and seed yield of European quinoa varieties. Environmental and Experimental botany, 177(1), 1-14.

- Sellami, M. H., Pulvento, C. & Lavini, A. (2021). Agronomic practices and performances of quinoa under field conditions: A systematic review. Plants, 10(72), 1-20.

- Shakeri, E., Emam, Y., Tabatabaei, S. & Sepaskhah, A. (2017). Evaluation of grain sorghum (Sorghum bicolor L.) lines/cultivars under salinity stress using tolerance indices. International Journal of Plant Production, 11(1), 101–116.

- Shen, J., Guo, M. J., Wang, Y. G., Yuan, X. Y., Wen, Y. Y., Song, X. E., Dong, Sh. Q. & Guo, P. Y. (2020). Humic acid improves the physiological and photosynthetic characteristics of millet seedlings under drought stress. Plant Signaling & Behavior, 5(8), 1-16.

- Shi, P. & GU, M. (2020). Transcriptome analysis and differential gene expression profiling of two contrasting quinoa genotypes in response salt stress. BMC Plant Biology, 20(1), 1-15.

- Tabatabaei, S. A. & Ansari, O. (2020). The effect of priming on germination characteristics of barley seeds under drought stress conditions. Cercetări Agronomice în Moldova, 53(1), 1-18.

- Wali, A., Salah, I. B., Zerrouki, M., Choukchou-Braham, A., Kamoun, Y. & Ksibi, M. (2019). A novel humic acid extraction procedure from Tunisian lignite. Euro-Mediterranean Journal for Environmental Integration, 4(1), 1-9.

- Wang, Sh., Wei, M., Wu, B., Cheng, H., Jiang, K. & Wang, C. (2020). Does N deposition mitigate the adverse impacts of drought stress on plant seed germination and seedling growth? Acta Oecologica, 109(1), 1-13.

- Xu, Sh., Zhao, N., Qin, D., Liu, Sh., Jiang, Sh., Xu, L., Sun, Z., Yan, D. & Hu, A. (2021). The synergistic effects of silicon and selenium on enhancing salt tolerance of maize plantsa. Environmental and Experimental Botany, 187(2), 104482.

- Zhang, W., Hao, Y., Teng, C., Fan, X., Yang, X., Liu, M., Ren, G. & Tan, C. (2020). Effects of salt stimulation on lunasin accumulation and activity during soybean germination. Foods, 9(2), 1-9.