Journal of Vegetables Sciences

Journal of Vegetables Sciences

Investigation of the Phenological Stages of Early Growth in Persian Leek (Allium ampeloprasum ssp. persicum)

Document Type : Original Article

Authors
Ayoub Ghorbani Dehkordi 1
Seyyed Javad Mousavizadeh 2
1 1- Assistant Professor, Department of Horticultural Sciences and Landscape, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 2- Associate Professor, Department of Horticultural Sciences and Landscape, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
10.22034/iuvs.2025.2068874.1421
Abstract
1. Introduction: Persian leek (Allium ampeloprasum ssp. persicum), a member of the Alliaceae family, is a traditional leafy vegetable widely used in Iranian cuisine. Its growth and development are strongly influenced by environmental factors, particularly temperature and light, which affect its morphology, physiology, and nutritional quality. Optimal germination and vegetative growth in Allium species generally occur at temperatures between 12°C and 24°C. While high temperatures above 30°C may inhibit early growth in some species like garlic, other studies report enhanced vegetative growth at around 30°C, indicating species-specific and cultivar-specific variability in thermal response. The determination of an appropriate planting date plays a critical role in aligning crop development with favorable environmental conditions, thus improving both the quantity and quality of yield. Given that climatic conditions vary regionally and that different cultivars respond differently to these conditions, it is essential to identify the best sowing time based on local climate, cultivar characteristics, and production goals. To date, most studies on Persian leek have focused on seed germination under controlled conditions and its physiological or nutritional properties. However, limited information is available on its phenological development and yield performance in open-field cultivation. Since the harvestable parts of Persian leek are its vegetative organs, understanding how different planting dates affect its early growth stages and final yield is essential. This study was conducted in Gorgan County, Golestan Province, over six months to monitor phenological stages and yield under varying planting dates, aiming to identify the optimal sowing time for Persian leek under local field conditions.
2. Materials and Methods: To investigate the phenological stages and yield of Persian leek under field conditions, the present study was conducted over 12 planting dates (one in each month of the year) with three replications at the research farm of Gorgan University of Agricultural Sciences and Natural Resources. Seed sowing was carried out on the 15th day of each month from Farvardin to Esfand (March to February). Daily minimum and maximum temperatures were recorded in the field at 8:00 AM, and the average temperature was calculated for each day and subsequently averaged monthly. Upon seedling emergence, phenological stages of the seedlings, including eight stages (emergence, hooked, green hooked, scape elongation, first true leaf appearance, first true leaf formation, second true leaf appearance, and second true leaf formation), were recorded according to the BBCH (Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie) scale. The date of occurrence for each phenological stage was recorded when 50% of the plants reached that stage. Yield measurements for each planting date were conducted monthly for six months. Finally, data were analyzed using SAS software version 9.4; mean comparisons were performed using Duncan’s multiple range test. Graphs were plotted using Microsoft Excel, and correlation analyses were performed with R software.
3. Results and Discussion: The results showed that the highest number of seedlings emerged per day occurred in July and August, with an average of 10.4 seedlings per day, followed by June and September with an average of 7.8 seedlings per day. The lowest number of seedlings emerged per day, with averages of 1.3 and 1.28, respectively, belonged to February and March. The highest number of seedlings entering the hook and green hook stages was also recorded in July and August, with an average of 7.8 seedlings per day. For the whip stage, the highest average daily number of seedlings entering this phase occurred in June with an average of 6.1 seedlings per day, and in July, August, and September with an average of 6.34 seedlings per day. Examination of the timing of phenological stages (days after planting) across different months revealed that the longest duration to reach the phenological stages of emergence, start of the hook stage, start of the green hook stage, and start of the whip stage was observed in January with an average temperature of 12.94 °C, while the shortest duration occurred in July and August with average temperatures of 29.37 °C and 30 °C, respectively. The highest number of seedlings entering the first true leaf emergence stage was recorded in June, July, August, and September, with average numbers of 5.27, 5.7, 5.7, and 5.27 seedlings per day, respectively. For the first true leaf formation stage, the highest numbers of seedlings entering this phase in July, August, and September were 3.9, 3.9, and 3.53 per day, respectively. The highest average numbers of seedlings entering the second true leaf emergence and formation stages per day were recorded in July and August, with values of 2.61 and 2.17, respectively. Based on the results, the shortest number of days after planting to reach the first and second true leaf emergence and formation stages occurred in July and August, whereas December exhibited the greatest delay in phenological development, followed by November. The highest harvestable organ yield was recorded in March and April with 1.12 and 1.07 kg/m², respectively, and the lowest yield was observed in November with 0.54 kg/m². Correlation analysis indicated that the emergence and formation stages of the first and second true leaves had a significant positive relationship with yield.
4. Conclusion: One of the effective strategies for optimal use of environmental factors, inputs, and increasing yield is to pay attention to the determination of planting dates and the selection of suitable cultivars. Based on the results obtained from the present study, it can be stated that under the conditions of Gorgan County, Golestan Province, the increase in temperature during March and April leads to the acceleration of phenological stages, which in turn increases the growth rate and ultimately enhances the final yield of the vegetative organs. In planting dates of November, December, and January, the emergence and formation of the first and second true leaves are delayed, and the growth period of Iranian leek is not completed. Conversely, in the planting dates of June, July, and August, due to the onset of intensified heat and water evaporation, leaf development stages are reduced. Therefore, the suitable planting dates for Iranian leek are March and April.
Keywords
Hook Stage
Emergence
Temperature
True leaf
Whip Stage
Akbari, S., Dashti, F., Gholami, M., & Arshadi, A. 2013. Evaluation of performance and some morphological and physiological characteristics of Iranian leek under salinity stress conditions. Iranian Journal of Horticultural Science and Technology, 14(2), 169-178. (In Persian)
Atif, M.J., Amin, B., Ghani, M.I., Ali, M., Zhang, S. & Cheng, Z. (2020). Effect of photoperiod and temperature on garlic (Allium sativum L.) bulbing and selected endogenous chemical factors. Environmental and Experimental Botany, 180, 104250.  https://doi.org/10.1016/j.envexpbot.2020.104250
Bareemizadeh, F., Karimi, N., Ghasempour, H.R., Maassoumi, S.M. & Taran, M. (2014). Essential oil composition of Allium ampeloprasum L. var. atroviolaceum and Allium iranicum. International Journal of Biosciences, 1(4), 372-377.
Bernatchez, A. & Lapointe, L. (2024). Cooler temperatures favour growth of wild leek (Allium tricoccum), a deciduous forest spring ephemeral. Botany, 90(12), 1125-1132. https://doi.org/10.1139/b2012-089
Bosekeng, G., & Coetzer, G.M. (2015). Response of onion (Allium cepa L.) to sowing date and plant population in the Central Free State, South Africa. African Journal of Agricultural Research, 10(4), 179-187. https://doi.org/10.5897/AJAR2013.8071
Carrizo, J.E. & Ibáñez, A.A. (2023). Phenology and production of garlic according to air and soil temperatures in La Rioja (Argentina). Horticultura Argentina, 42, 70-84.
Czóbel, S., Saláta, D., Baltazár, T., Trenyik, P. & Szirmai, O. (2023). Examining the Stand Level CO2 Fluxes of Spring Forest Geophytes. Forests, 14(5), 860. https://doi.org/10.3390/f14050860
Dashti, F. (2003). The study of genetic diversity and phylogeny of Tareh Irani in Alliums using morphological characters and molecular markers. Ph. D. Thesis in Horticultural Science, Faculty of Agriculture, University of Tehran.
El-Keblawy, َ. & Al-Rawai, A. (2005). Effects of salinity, temperature and light on germination of invasive Prosopis juliflora. Journal of Arid Environments, 61(4), 555-565. https://doi.org/10.1016/j.jaridenv.2004.10.007   
Figliuolo, G. & Di Stefano, D. (2007). Is a single bulb producing garlic Allium sativum or Allium ampeloprasum? Scientia Horticulturae, 114, 243–249. https://doi.org/10.1016/j.scienta.2007.06.021
Gorai, M., Moussaa, A. & Neffati, M. (2016). Does mineral sulphur availability account for growth performance, bulb development and metabolically related traits in wild leek  (Allium ampeloprasum  L.;  Alliaceae)? Flora, 219, 8-17. https://doi.org/10.1016/j.flora.2015.12.003
Guenaoui, C., Ksiksi Taoufik, S., Smiti, S. & Neffati, M. (2012). Response of seed germination of Tunisian Allium ampeloprasum to temperature and salt stresses. Revue d’Écologie, 67, 399-408. https://doi.org/10.3406/revec.2012.1654
Harding, S. (2005). Inflorescence development in Allium ampeloprasum var. babingtonii (Babington’s leek) - ProQuest. Cardiff University (United Kingdom) ProQuest Dissertations & Theses.
Jafari, H. & Delshad, M. (2019). Determination of cardinal temperatures for seed germination of Iranian leek (Allium ampeloprasum ssp. persicum) and parsley (Petroselinum crispum). The First Conference on Fundamental Research in Agricultural and Environmental Sciences, June 12, 2019, Tehran, Iran. (In Persian)
Kamboj, N. K., Batra, V.K., Aniket Vilas, C.A., & Sharma, P.K. (2017). Effect of planting dates and paclobutrazol on yield and quality of onion (Allium cepa L.) seed. International Journal Pure Apply Bioscience, 5(1), 417-424. https://doi.org/10.18782/2320-7051.2457
Karimi, N. & Saiedikhah, Z. (2018). Effect of selenium on growth and some physiological parameters of Allium iranicum Wendelbo. and Allium ampeloprasum L. Journal of Plant Process and Function, 7, 183-198. https://doi.org/10.1001/1.23222727.1397.7.24.1.6
Karimzadeh Soureshjani, H., Bahador, M., Tadayon, M. & Ghorbani Dehkordi, A. (2019). Modelling seed germination and seedling emergence of flax and sesame as affected by temperature, soil bulk density, and sowing depth. Industrial Crops & Products, 141, 1–8. https://doi.org/10.1016/j.indcrop.2019.111770
Khade, Y., Thangasamy, A. & Gorrepati, K. (2017). Garlic production technology. Indian Horticulture, 62, 57-59.
Krontal, Y., Kamenetsky, R., & Rabinowitch, H.D. (2000). Flowering physiology and some vegetative traits of short-day shallot: a comparison with bulb onion. Journal of Horticultural Science and Biotechnology, 75(1), 35-41. https://doi.org/10.1080/14620316.2000.11511197
Mallek, S.B., Prather, T.S. & Stapleton, J.J. (2007). Interaction effects of Allium spp. residues, concentrations and soil temperature on seed germination of four weedy plant species. Applied Soil Ecology, 37, 233-239. https://doi.org/10.1016/j.apsoil.2007.07.003
Mekonnen, A. & Gadisa, N. (2021). Agronomic practices for improving garlic (Allium sativum L.) production and productivity in Ethiopia Review. World Journal of Agricultural Sciences, 6(17), 469-477. https://doi.org/10.5829/idosi.wjas.2021.469.477
Parida, A.K. & Das, A.B. (2005). Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60, 324–349. https://doi.org/10.1016/j.ecoenv.2004.06.010
Samarah, N. & Abu-Yahya, A. (2008). Effect of maturity stages of winter-and spring-sown chickpea (Cicer arietinum L.) on germination and vigor of the harvested seeds. Seed science and technology, 36(1), 177-190. https://doi.org/10.15258/sst.2008.36.1.19
Shahmansouri, E., Abasi, Z. & Shirazi, F. (2021). Study the Effect of Harvesting Time and Drying Method on Allium cepa Seed Germination Quality. Journal of Vegetables Sciences, 4(8), 133-145. https://doi.org/10.22034/iuvs.2021.130022.1111
Shahmansouri, E. & Haghighi, M. (2022). Optimization of Dry Onion Sets Production in Long Day and Short Day Types. Journal of Vegetables Sciences, 5(10), 151-162. https://dor.isc.ac/dor/20.1001.1.26764814.1400.5.2.10.2
Tchórzewska, D., Bocianowski, J., Najda, A., Dąbrowska, A. & Winiarczyk, K. (2017). Effect of environment fluctuations on biomass and allicin level in Allium sativum (cv. Harnas, Arkus) and Allium ampeloprasum var. ampeloprasum (GHG-L). Journal of Applied Botany and Food Quality, 90, 106-114. https://doi.org/DOI:10.5073/JABFQ.2017.090.013
Ulianych, O., Yatsenko, V., Didenko, I., Vorobiova, N., Kuhnyuk, O., Lazariev, O.  & Tretiakova, S., 2019. Agrobiological evaluation of Allium ampeloprasum L. variety samples in comparison with Allium sativum L. cultivars. Agronomy Research, 17(4), 1788-1799. https://doi.org/10.15159/AR.19.192
Yamaguchi, M., Paulson, K.N., Kinsella, M.N. & Bernhard, R.A. (1975). Effects of Soil Temperature on Growth and Quality of Onion Bulbs (Allium cepa L.) Used for Dehydration1. ResearchGate. https://doi.org/10.21273/JASHS.100.4.415  
Journal of Vegetables Sciences
Volume 10, Issue 19
July 2026
Pages 63-82

  • Receive Date 14 August 2025
  • Revise Date 22 September 2025
  • Accept Date 29 September 2025