دو فصلنامه علوم سبزی ها

دو فصلنامه علوم سبزی ها

بررسی اثرات کشندگی حشره‌کش اسپیروتترامات، روغن سیتووت و مخلوط آن‌ها علیه آفت سفید بالک (Bemisia tabaci) روی گوجه‌فرنگی در شرایط گلخانه

نوع مقاله : مقاله پژوهشی

نویسنده
استادیار بخش تحقیقات گیاه‌پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان مرکزی، سازمان تحقیقات، آموزش و ترویج کشاورزی، اراک، ایران
چکیده
گوجه‌فرنگی نقش مهمی در اقتصاد گلخانهداران دارد. سفیدبالک (Bemisia tabaci Gennadius.) یکی از مهمترین آفات محصولات گلخانه‌ای کشور است که از آفات کلیدی گوجه‌فرنگی نیز به‌شمار می‌رود. یکی از متداولترین روشهای کنترل این آفت، استفاده از آفت‌کشها میباشد ولی عوامل زیستی مختلف مانند دامنه وسیع میزبانی، چند نسلی بودن، نرخ بالای تولیدمثل، توانایی انتقال ویروسهای گیاهی و گسترش مقاومت به حشرهکشها باعث مشکلاتی در مدیریت این آفت شده است. بنابراین استفاده از حشرهکشهای جدید جهت کنترل این آفت ضروری به‌نظر میرسد. در این پژوهش حساسیت مراحل مختلف زیستى سفیدبالک جالیز Bemisia tabaci نسبت به اسپیروتترامات، روغن سیتووت و مخلوط آنها مورد ارزیابی قرار گرفت. از روغن سیتووت و اسپیروتترامات در پنج غلظت و شاهد استفاده شد. مقدار LC50 حشرهکش اسپیروتترامات براى حشره‌ی بالغ، تخم، مراحل نابالغ به‌ترتیب 53/5، 10/60 و46/1 پى‏پى‏ام تعیین گردید. LC50 روغن سیتووت براى حشرهی بالغ، تخم، مراحل نابالغ به‌ترتیب 1743، 62/977 و 36/728 پى‏پى‏ام برآورد شد. اسپیروتترامات و روغن سیتووت اثر بیشتری روی مراحل نابالغ نسبت به سایر مراحل داشتند. به‌منظور ارزیابى نقش سینرژیستى روغن سیتووت LC25 اسپیروتترامات در هر مرحله زیستى باLC25 روغن سیتووت مخلوط گردید که در نتیجه میزان تلفات در حشره‏ بالغ، تخم و مراحل نابالغ به‌ترتیب 77/54، 55/61 و 65/68 درصد به‌دست آمد و مشخص گردید روغن سیتووت اثر اسپیروتترامات را تشدید مى‏کند. با توجه به قیمت کمتر روغن سیتووت نسبت به اسپیروتترامات، تأثیر افزایشی آن بر حشرهکش و همچنین سازگاری بیشتر این روغن با محیط زیست، استفاده از مخلوط آنها برای کنترل این آفت قابل‌توصیه میباشد.
کلیدواژه‌ها

عنوان مقاله English

Investigation of lethal effects of spirotetramat, citowett oil and their mixture against Bemisia tabaci on tomato in greenhouse conditions.

نویسنده English

Sedighe Ashtari
Assistant Professor, Plant Protection Research Department, Markazi Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization, AREEO, Arak, Iran
چکیده English

Introduction: Bemisia tabaci is known in Iran and many other countries as a first-class Cotton pest. This pest is one of the most important pests of Tomatoes in greenhouses as well as other crops, summer crops and greenhouses in most parts of the world, especially in hot, temperate, and dry regions of the world. Due to the high damage caused by this pest, Tomato greenhouses are frequently sprayed during the growing season.
Materials and Methods: Plastic pots with a diameter of 18 and a height of 18 cm were selected. Tomato seeds were planted in pots. After the Tomato plants sprouted, the weak plants were removed from the pots. For immature preparation, large numbers of adult insects were released on Tomato pots and observed on the leaves four weeks after release of all biological stages. To prepare the eggs, large numbers of white cotton bulb insects were released on tomato plants planted in pots. For this purpose specimens were collected from contaminated Tomato flowerpots. After immersion of leaves in the pesticide solutions, the sensitivity of different life stages was evaluated. The purpose of this test is to determine the range of concentrations of insecticides that had been led to 25-75 percent of death. The main concentrations were calculated by the concentration of preliminary experiments. Likewise, control mortality was corrected using Abbott's formula.
Results and Discussion: The results of this study, according to the average lethal concentration of 50 percent of the insecticide Spirotetramat showed that this insecticide was evaluated toxic for immature, adult and eggs, respectively. Since spirotetramat is a systemic insecticide and also feeds the adult and immature stages of this pest, adult and immature stages are more sensitive than eggs. The reason for the less susceptibility of eggs to this insecticide is the presence of an impermeable layer of chorion. Due to the way spirotetramat works, it especially affects the immature stages of sucking pests. In the case of adult female insects, this compound significantly reduces fertility and reproduction, resulting in a decrease in insect population. Gulen et al. investigated the cross-resistance and susceptibility of Western flower thrips to spirotetramat in vitro on bean pods and reported that spirotetramat was more toxic to the nymph stages of the insect tested (Guillen et al., 2014). The results of this study are similar to the results of the present study in terms of the greater effect of spirotetramat on the immature stage (Gaskin et al., 2010) in field experiments reported that the addition of an oil and dispersant to the insecticide spirotetramat had a good effect on controlling oysters. These oysters are generally very difficult to control due to the hard shell of the insect because insecticide alone cannot penetrate. The results of this study are similar to the results of the present study, which shows the increased effect of spirotramat by citowett oil.

Bioassay with citowett oil showed that immature stages and eggs were more sensitive than adult insects. Which can be due to the contact toxicity of the oil. To evaluate the effect of spirotetramat and citowett oil insecticide mixture, their LC25 doses were mixed and the effect was determined on different stages and the resulting mortality rate was determined. The mortality rate due to the mixture of LC25, insecticide spirotetramate and citutot oil was higher than the total expected losses (50%), 4.77% in adult insects, 11.55% in egg stage and 18.65% in immature stage. The increase in lethality after adding oil to the insecticide is consistent with the results obtained by some researchers (Ashtari, 2019; Ashtari et al., 2020; Hosseini Naveh et al., 2010).

Conclusions: Based on the results of the study and the studies of other researchers, it can be concluded that the white-tailed deer, due to its special biological and behavioral conditions, has a high potential for resistance to most toxins. Applying proper management principles to control the mass of its population should be done. The issue of the phenomenon of resistance to toxins has accelerated in it.

کلیدواژه‌ها English

Bemisia tabaci
Bioassay
Citowett oil
Spirotetramat Insecticide
Synergism
  • Abbott, W. S. (1925). A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18(2), 265-267.
  • Al-Dosary, M. M., Al-Bekairi, A. M. & Moursy, E. B. (2010). Orphology of the egg shell and the developing embryo of the Red Palm Weevil, Rhynchophorus ferrugineus (Oliver). Saudi Journal Biological Sciences, 17(2), 177-183.
  • Arjmandinejad, A. & Sheikhi Gorjan, A. (2011). Investigation of the effect of new insecticides on Bemisia tabaci and Trialeurodes vaporariarum. Second National Conference on Pest Management, September 14, Kerman, 311-314.
  • Ashtari, S. (2017). Investigation of effectiveness of Pyriproxyfen and Citowett oil in control of Bemisia tabaci. Journal of Plant Protection, 31(4), 639-644. (In Persian)
  • Ashtari, S. (2019). Control of tomato leaf mine moth using chemical pesticides and beneficial insects, Journal of Greenhouse Vegetable Extension, 1(1), 33-39. (In Persian)
  • Ashtari, S., Sabhi, G. & Talebi Jahromi, K. (2020). Effects of four insecicides on immature stages of Trichogramma evanescens Westwood (Hymenoptera: Trichogrammatidae) Egg parasitoid of Tuta absoluta (Meyrick) (Lep: Gelechiidae). Journal of Applied Research in Plant Protection, 9(1), 45-58. (In Persian)
  • Barbosa, P. R., Michaud, J. P., Bain, C. L. & Torres, J. B. (2017). Toxicity of three aphicides to the generalist predators Chrysoperla carnea (Neuroptera: Chrysopidae) and Orius insidiosus (Hemiptera: Anthocoridae). Ecotoxicology, 26(5), 589-599.
  • Bruck, E., Elbert, A., Fischer, R., Krueger, S., Kuhnhold, J., Klueken, A. M. & van Waetermeulen, X. (2009). Movento®, an innovative ambimobile insecticide for sucking insect pest control in agriculture: biological profile and field performance. Crop Protection, 28(10), 838-844.
  • Fernandes, F. L., Bacci, L. & Fernandes, M. S. (2010). Impact and selectivity of insecticides to predators and parasitoids. EntomoBrasilis, 3(1), 1-10.
  • Flint, M. L. (2002). Pest notes: Whiteflies. Oakland: University of California. Division of Agriculture and Natural Resources, Publication.
  • Francesena, N., Haramboure, M., Smagghe, G., Stadler, T. & Schneider, M. I. (2012). Preliminary studies of effectiveness and selectivity of Movento on Bemisia tabaci and its parasitoid Eretmocerus mundus. Communications in Agricultural and Applied Biological Sciences, 77(4), 727-733.
  • Gaskin, R. E., Horgan, D. B. & Manktelow, D. W. (2010). Adjuvant effects on the retention and uptake of spirotetramat insecticide sprays on kiwifruit. New Zealand Plant Protection, 63, 60-65.
  • Goroohi, F., Imani, S., Samih, M. A. & Panahi, B. (2018). Effects of ethanolic extracts of Rubia tinctorum, Ferula gummosa and Nesidiocoris tenuis (Hem.: Miridae) on sweet potato whitefly, Bemisia tabaci (Hem.: Aleyrodidae), and comparison with Spirotetrmat. IAU Entomological Research Journal, 10(2), 111-125.
  • Guillen, J., Navarro, M. & Bielza, P. (2014). Cross-resistance and baseline susceptibility of spirotetramat in Frankliniella occidentalis (Thysanoptera: Thripidae). Journal of Economic Entomology, 107(3), 1239-1244.
  • Hesselbach, H. & Scheiner, R. (2019). The novel pesticide flupyradifurone (Sivanto) affects honeybee motor abilities. Ecotoxicology, 28(3), 354-366.
  • Hopkinson, J., Pumpa, S., van Brunschot, S., Fang, C., Frese, M., Tay, W. T. & Walsh, T. (2020). Insecticide resistance status of Bemisia tabaci MEAM1 (Hemiptera: Aleyrodidae) in Australian Cotton production valleys. Austral Entomology, 59(1), 202-214.
  • Hosseini Naveh, F., Poormirza, A. A. & Safaralizadeh, M. H. (2010). Evaluation of effects of primicarb, citowett oil and the mixture of either compound on Trialeurodes vaporariorum and Myzus persicae in greenhouse. Iran Plant Protection Science, 41(1), 95-101. (In Persian)
  • Hosseininia, A., Khanjani, M., Khoidel, M. & Javadikhedri, S. (2016). Comparision of the efficiency of the current oils and insecticide compounds in control of greenhouse whitefly, Trialeurodes vaporariorum (Westwood), (Hem: Aleyrodidae) on rose and their interaction, Journal of Plant Protection, 4(4), 718-726. (In Persian)
  • Malekmohammadi, A., Shishehbor, P. & Kocheili, F. (2012). Influence of constant temperatures on development, reproduction and life table parameters of Encarsia inaron (Hymenoptera: Aphelinidae) parasitizing Neomaskellia andropogonis (Hemiptera: Aleyrodidae). Crop Protection, 34, 1-5. (In Persian)
  • Mario, B., Giuliani, G., Tolotti, G. & Angeli, G. (2012). Efficacy of spirotetramat on Apple Aphids. Conference. Giornate Fitopatologiche Italiane, 1, 149-154.
  • Nauen, R., Reckmann, U., Thomzik, J. & Thielert, W. (2008). Biological profile of spirotetramat (Movento®)–a new two-way systemic (ambimobile) insecticide against sucking pest species. Bayer Crop Science Journal, 61(2), 245-278.
  • Nauen, R., Jeschke, P., Velten, R., Beck, M. E., Ebbinghaus‐Kintscher, U., Thielert, W. & Raupach, G. (2015). Flupyradifurone: a brief profile of a new butenolide insecticide. Pest Management Science, 71(6), 850-862.
  • Nazari, P., Poorjavad, N., Izadi, H. & Sahafi, S. R. (2020).Comparison of the effect of several common insecticides on Lepidosaphes malicola (Hem .: Diaspididae) in Semirom apple orchards, Plant Pest Research, 1(10), 17-30. (In Persian)
  • Ouyang, Y., Montez, G. H., Liu, L. & Grafton‐Cardwell, E. E. (2012). Spirodiclofen and spirotetramat bioassays for monitoring resistance in citrus red mite, Panonychus citri (Acari: Tetranychidae). Pest Management Science, 68(5), 781-787.
  • Page-Weir, N. E. M., Jamieson, L. E., Chhagan, A., Connolly, P. G. & Curtis, C. (2011). Efficacy of insecticides against the tomato/potato psyllid (Bactericera cockerelli). New Zealand Plant Protection, 64, 276-281.
  • Palumbo, J. C. (2011). Influence of adjuvants and spray timing of Movento on aphid contamination and crop injury in baby spinach. Plant Health Progress, 12(1), 10.
  • Peng, Z., Zheng, H., Xie, W., Wang, S., Wu, Q. & Zhang, Y. (2017). Field resistance monitoring of the immature stages of the whitefly Bemisia tabaci to spirotetramat in China. Crop Protection, 98, 243-247.
  • Prabhaker, N., Castle, S. J., Buckelew, L. & Toscano, N. C. (2008). Baseline susceptibility of Bemisia tabaci B biotype (Hemiptera: Aleyrodidae) populations from California and Arizona to spiromesifen. Journal of Economic Entomology, 101(1), 174-181.
  • Ranjbar Aghdam, H., Kamali, H., Foruzam, M. & Emami, M. S. (2018). Studying on the efficacy of spirotetramat (Movento SC 10%) for chemical control of the mussel scale, Lepidosaphes malicola. 23rd Iranian Plant Protection Congress, Tehran, Iran.
  • Robertson, J., Russell, R., Presiler, H. & Savin, N. E. (2007). Pesticide Bioassays with Arthropods. CRC Press, Inc.
  • Sahraeyan, H. (2016). Management of harmful factors in vegetables and weeds. extension magazine of Fars Province Jihad Agricultural Organization, 1-45. (In Persian)
  • Silva, E. A. I. & Murguido Morales, C. A. (2010). Spirotetramat, nuevo insecticida para el control de insectos chupadores en el cultivo de la papa. Fitosanidad, 14(4), 229-234.
  • Soleymanzade, A. (2014). Survey of effects of pesticides Proteus, Deltamethrin, Tiacloprid and their synergistic effect to Citowett oil on Trialeurodes vaporariorum. Thesis of MSc, University of Urmia. (In Persian)
  • Talebi Jahromi, K. H. (2013). Pesticides toxicology. Tehran University Press. (In Persian)
  • Vieira, S. S., Boff, M. I. C., Bueno, A. F., Gobbi, A. L., Lobo, R. V. & de Freitas Bueno, R. C. O. (2012). Effects of insecticides used in Bemisia tabaci (Gennadius) biotype B control and their selectivity to natural enemies in soybean crop. Semina: Ciencias Agrarias, 33(5), 1809-1818.
  • Willmott, A. L. (2012). Efficacy of systemic insecticides against the citrus mealybug, Planococcus citri, and pesticide mixtures against the western flower thrips, Frankliniella occidentalis. Ph.D dissertation, Faculty of protected environments, Kansas State University.
  • Yao, F. L., Zheng, Y., Huang, X. Y., Ding, X. L., Zhao, J. W., Desneux, N. & Weng, Q. Y. (2017). Dynamics of Bemisia tabaci biotypes and insecticide resistance in Fujian province in China during 2005–2014. Scientific Reports, 7(1), 1-12.

 

دوره 5، شماره 10 - شماره پیاپی 2
دو فصلنامه علوم سبزی ها- پاییز و زمستان 1400
دی 1400
صفحه 105-119

  • تاریخ دریافت 20 مرداد 1400
  • تاریخ بازنگری 04 مهر 1400
  • تاریخ پذیرش 15 آبان 1400